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Rabbit Anti-BPNT1  antibody (bs-20136R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
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订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-20136R
英文名称 BPNT1
中文名称 二磷酸3核苷酸酶抗体
别    名 4-phosphatase; Bisphosphate 3 prime nucleotidase; Bpnt1; BPNT1_HUMAN; BPntase; PAP inositol 1,4 phosphatase; PAP-inositol-1; PIP.  
研究领域 心血管  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat,Cow)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 33kDa
细胞定位 细胞浆 细胞外基质 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BPNT1: 2-100/308 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 BPNT1 is a member of the magnesium-dependent, lithium-sensitive phosphomonoesterase superfamily. Using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3’-phosphate 5’ phosphosulfate) to APS (adenosine 5’-phosphosulfate) and the conversion of PAP (3’(2’)-phosphoadenosine 5’ phosphate) to AMP (adenosine 5’-phosphate). Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Inhibition of BPNT1 leads to an accumulation of PAP and subsequent inhibition of sulfotransferases which may result in changes in gene expression, changes in phosphatidylinositol second messenger function and/or changes in sulfation processes.

Function:
Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6.

Tissue Specificity:
Highly expressed in kidney, liver, pancreas and heart. Detected at lower levels in brain, placenta, lung and skeletal muscle.

Similarity:
Belongs to the inositol monophosphatase family.

SWISS:
O95861

Gene ID:
10380

Database links:

Entrez Gene: 10380 Human

Omim: 604053 Human

SwissProt: O95861 Human

Unigene: 406134 Human



产品图片
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- BPNT1 (bs-20136R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti- BPNT1 (bs-20136R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Tissue/cell: Mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BPNT1 Polyclonal Antibody, Unconjugated(bs-20136R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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