产品编号 | bs-20136R |
英文名称 | BPNT1 |
中文名称 | 二磷酸3核苷酸酶抗体 |
别 名 | 4-phosphatase; Bisphosphate 3 prime nucleotidase; Bpnt1; BPNT1_HUMAN; BPntase; PAP inositol 1,4 phosphatase; PAP-inositol-1; PIP. |
研究领域 | 心血管 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse (predicted: Rat,Cow) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 33kDa |
细胞定位 | 细胞浆 细胞外基质 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BPNT1: 2-100/308 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
BPNT1 is a member of the magnesium-dependent, lithium-sensitive phosphomonoesterase superfamily. Using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3’-phosphate 5’ phosphosulfate) to APS (adenosine 5’-phosphosulfate) and the conversion of PAP (3’(2’)-phosphoadenosine 5’ phosphate) to AMP (adenosine 5’-phosphate). Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Inhibition of BPNT1 leads to an accumulation of PAP and subsequent inhibition of sulfotransferases which may result in changes in gene expression, changes in phosphatidylinositol second messenger function and/or changes in sulfation processes. Function: Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6. Tissue Specificity: Highly expressed in kidney, liver, pancreas and heart. Detected at lower levels in brain, placenta, lung and skeletal muscle. Similarity: Belongs to the inositol monophosphatase family. SWISS: O95861 Gene ID: 10380 Database links: Entrez Gene: 10380 Human Omim: 604053 Human SwissProt: O95861 Human Unigene: 406134 Human |
产品图片 |
Sample:
Pancreas (Mouse) Lysate at 40 ug Primary: Anti- BPNT1 (bs-20136R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Sample:
U251(Human) Cell Lysate at 30 ug Primary: Anti- BPNT1 (bs-20136R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Tissue/cell: Mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BPNT1 Polyclonal Antibody, Unconjugated(bs-20136R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |