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Rabbit Anti-ADRB1  antibody (bs-20177R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-20177R
英文名称 ADRB1
中文名称 肾上腺素能受体β1抗体
别    名 beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; Beta 1 adrenoceptor; Beta 1 adrenoreceptor; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1.  
Specific References  (2)     |     bs-20177R has been referenced in 2 publications.
[IF=5.955] Nguyen Khanh Toan. et al. Ascorbic acid induces salivary gland function through TET2/acetylcholine receptor signaling in aging SAMP1/Klotho (-/-) mice.. AGING-US. 2022 Aug;14(15):6028-6046  WB ;  Mouse.  
[IF=3.329] Enivwenaye E.W. Nabofa et al. Cardiovascular Effects of Caffeine in Rabbits Involve Beta-1 Adrenergic Receptor Activation.  IHC ;  rabbit.  
研究领域 心血管  神经生物学  信号转导  生长因子和激素  通道蛋白  细胞膜受体  细胞膜蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Pig)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 52kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ADRB1: 181-250/477 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family.

Function:
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.

Subunit:
Interacts with GOPC, MAGI3 and DLG4.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.

Post-translational modifications:
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Similarity:
Belongs to the G-protein coupled receptor 1 family.
Adrenergic receptor subfamily. ADRB1 sub-subfamily.

SWISS:
P08588

Gene ID:
153

Database links:

Entrez Gene: 153 Human

Entrez Gene: 11554 Mouse

Entrez Gene: 24925 Rat

Omim: 109630 Human

SwissProt: P08588 Human

SwissProt: P34971 Mouse

SwissProt: P18090 Rat

Unigene: 99913 Human

Unigene: 46797 Mouse

Unigene: 87064 Rat



产品图片
Sample:
K562 (human)cell Lysate at 40 ug
HT29 (human)cell Lysate at 40 ug
Primary: Anti- ADRB1 (bs-20177R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 62 kD
Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti-ADRB1 (bs-20177R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Sample: 293T (human)cell Lysate at 40 ug
Primary: Anti- ADRB1 (bs-20177R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 62 kD
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Primary: Anti-ADRB1 (bs-20177R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADRB1) Polyclonal Antibody, Unconjugated (bs-20177R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (bs-20177R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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