| 产品编号 | bs-8009R |
| 英文名称 | ADAMTS6 Rabbit pAb |
| 中文名称 | 整合素样金属蛋白酶与凝血酶样6蛋白抗体 |
| 别 名 | A Disintigrin And Metalloproteinase with ThromboSpondin motif 6; ADAMTS 6; Thrombospondin domain 2.ATS6_HUMAN |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 97 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAMTS6: 231-330/1117 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. [provided by RefSeq, Jul 2008] Function: ADAMTS6 was first described along with ADAMTS5 and ADAMTS7 in database searches for proteases containing the metalloprotease domain signature. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the function of ADAMTS6 is still poorly understood, and as of this writing no substrates have been shown to be cleaved by ADAMTS6. ADAMTS6 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and is likely to be an active protease. In addition to the metalloprotease domain, ADAMTS6 has a propeptide domain, a Prohormone Convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain and five thrombospondin 1 like domains. The domain structure of ADAMTS6 is thus most like ADAMTS16 and ADAMTS19. ADAMTS6 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein. Other ADAMTSs family members (ADAMTS4, ADAMTS5) have been more thoroughly studied, and are known to play key roles in aggrecan turnover. Analysis of breast cancer cell lines and RPE cells in the eye show elevated expression of ADAMTS6, and the original paper cited low levels of expression in the placenta. Three human sequences are currently reported, 1117, 860 and 468 amino acids in length respectively. The 860 amino acid sequence has a shorter spacer after the catalytic domain, and ends in the second thrombospondin domain. The 468 amino acid sequence ends at the start of the disintegrin domain. It remains to be seen where these different splice variants are expressed. The 1117 amino acid protein has a predicted mass of 125.27 kDa, the 860 form 97.11 kDa and the 468 form 53.12 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS6 a greater apparent molecular weight on reduced SDS PAGE gels. The furin consensus motif in ADAMTS6 means that the protein is likely to be cleaved when it is secreted. Subcellular Location: Secreted protein; extracellular space; extracellular matrix. Tissue Specificity: Expressed at low levels in placenta and barely detectable in a number of other tissues. Post-translational modifications: The precursor is cleaved by a furin endopeptidase. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). DISEASE: Isoform 1 and isoform 2 expressions are up-regulated by TNF in retinal pigment epithelial cells. Similarity: Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 1 PLAC domain. Contains 5 TSP type-1 domains. SWISS: Q9UKP5 Gene ID: 11174 Database links: Entrez Gene: 11174 Human Entrez Gene: 108154 Mouse Omim: 605008 Human SwissProt: Q9UKP5 Human Unigene: 482291 Human Unigene: 208125 Mouse |
| 产品图片 |
Sample:
U251 (Human)CellLysate at 40 ug
Hela (Human) CellLysate at 40 ug
Primary: Anti-ADAMTS6(bs-8009R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 97kD
Observed band size: 125kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADAMTS6) Polyclonal Antibody, Unconjugated (bs-8009R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
