产品编号 | bs-10815R |
英文名称 | Rabbit Anti-FMDV VP1 antibody |
中文名称 | 口蹄疫病毒VP1抗体 |
别 名 | foot and mouth disease virus; Foot-and-mouth disease virus-type O; Foot-and-mouth disease virus-type Asia 1; Foot-and-mouth disease virus-type Asia 1; Foot-and-mouth disease virus-type C; VP1 protein; FMDV Polyprotein (VP1 protein). |
研究领域 | 细菌及病毒 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | FMDV |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 240kDa |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Recombinant FMDV Asia1 VP1 (His tag) |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The foot-and-mouth disease virus (FMDV) is the pathogen that causes foot-and-mouth disease. It is a picornavirus, the prototypical member of the Aphthovirus genus. The disease, which causes vesicles (blisters) in the mouth and feet of bovids, suids, ovids, caprids and other cloven-hoofed animals is highly infectious and a major plague of animal farming. The virus particle (25-30 nm) has an icosahedral capsid made of protein, without envelope, containing a single strand of ribonucleic acid (RNA) containing a positive encoding of its genome. When the virus comes in contact with the membrane of a host cell, it binds to a receptor site and triggers a folding-in of the membrane. Once the virus is inside the host cell, the capsid dissolves, and the RNA gets replicated, and translated into viral proteins by the cell's ribosomes using a cap-independent mechanism driven by the internal ribosome entry site element. The foot-and-mouth disease virus occurs in seven major serotypes: O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1. These serotypes show some regionality, and the O serotype is most common. Subcellular Location: Host cytoplasm. SWISS: N/A Gene ID: N/A |
产品图片 |
Sample:
Lane 1: Recombinant FMDV VP1 protein, Sumo & His(bs-10815P)
Lane 2: Recombinant FMDV VP1 (type O) protein, Sumo & His(bs-41028P)
Lane 3: Recombinant FMDV VP1 (type O) protein, Sumo & His(bs-41049P)
Primary: Anti-FMDV VP1 (bs-10815R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 240 kDa
Observed band size: 45 kDa
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |