产品编号 | bs-21082R |
英文名称 | OLFML1 |
中文名称 | 嗅球蛋白样蛋白1抗体 |
别 名 | MVAL564; Olfactomedin like 1; Olfactomedin like protein 1; OLFML 1; OLFML1; UNQ564. |
Specific References (1) | bs-21082R has been referenced in 1 publications.
[IF=4.174] Zhang, Zhao. et al. Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer. Hum Cell. 2022 Jan;:1-16 IHC ; Human.
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研究领域 | 细胞生物 神经生物学 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse (predicted: Human,Rat,Dog,Horse,Rabbit,Sheep) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 46kDa |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human OLFML1: 321-402/402 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
OLFML1 (Olfactomedin-like protein 1) contains 1 olfactomedin-like domain. The specific function of this protein remains unknown. Subcellular Location: Secreted. SWISS: Q6UWY5 Gene ID: 283298 Database links: Entrez Gene: 283298 Human Entrez Gene: 244198 Mouse SwissProt: Q6UWY5 Human |
产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Eye (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- OLFML1 (bs-21082R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML1) Polyclonal Antibody, Unconjugated (bs-21082R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |