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Rabbit Anti-QPRT/QAPRTase  antibody (bs-20316R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-20316R
英文名称 QPRT/QAPRTase
中文名称 喹啉酸磷酸核糖基转移酶抗体
别    名 NADC_HUMAN; Nicotinate nucleotide pyrophosphorylase (carboxylating); Nicotinate-nucleotide pyrophosphorylase [carboxylating]; QAPRTase; QPRT; QPRTase; Quinolinate phosphoribosyltransferase [decarboxylating]; Quinolinate phosphoribosyltransferase.  
研究领域 肿瘤  细胞生物  神经生物学  信号转导  Alzheimer's  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse (predicted: Sheep,Cow,Dog,Human)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 31kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human QPRT/QAPRTase : 21-120/297 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Quinolinate phosphoribosyltransferase (QPRTase) is a major enzyme in the catabolism of quinolinate. Quinolinate is an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide, and pyrophosphate. Catabolism of quinolinate is vital due to the quinolinate’s neurotoxicity. Increased levels of quinolinate have been linked to neurodegenerative symptoms associated with meningitis and AIDS. QAPRTase has a seven-stranded alpha/beta-barrel domain which is similar in structure to the eight-stranded alpha/beta-barrel enzymes. The protein possesses a novel fold in comparison to other members of the PRTase family. This fold comprises a structure combining two domains. The structure is part alpha/beta barrel-like domain, and part alpha/beta N-terminal domain.

Function:
Involved in the catabolism of quinolinic acid (QA).

Subunit:
Hexamer formed by 3 homodimers.

Similarity:
Belongs to the nadC/modD family.

SWISS:
Q15274

Gene ID:
23475

Database links:

Entrez Gene: 614254 Cow

Entrez Gene: 607664 Dog

Entrez Gene: 100727900 Guinea pig

Entrez Gene: 100065768 Horse

Entrez Gene: 23475 Human

Entrez Gene: 100406642 Marmoset (common)

Omim: 606248 Human

SwissProt: Q3T063 Cow

SwissProt: Q15274 Human

Unigene: 513484 Human



产品图片
Sample: Liver (mouse) Lysate at 40 ug
Primary: Anti- QPRT'QAPRTase (bs-20316R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31kD
Observed band size: 31kD
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Lane 3: Mouse Kidney tissue lysates
Primary: Anti-QPRT/QAPRTase (bs-20316R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 28 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Quinolinate phosphoribosyltransferase; QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (bs-20316R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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