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Rabbit Anti-Leptin receptor  antibody (bs-20498R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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产品编号 bs-20498R
英文名称 Leptin receptor
中文名称 瘦素受体抗体
别    名 obl; CD 295; CD295; CD295 antigen; Db; Fa; HuB219; LEP R; LEPR; LEPROT; Leptin receptor fatty; Leptin receptor gene related protein; Leptin receptor precursor; OB R; OB-R; OB R gene related protein; OB receptor; OB-RGRP; Obr; Ob-R a/b/c/d/e; LEPR_HUMAN; LEPRD; Leptin receptor.  
Specific References  (1)     |     bs-20498R has been referenced in 1 publications.
[IF=1.227] Ding YY et al. Relationship among porcine lncRNA TCONS_00010987, miR-323, and LEPR based on dual luciferase reporter gene assays and expression patterns. Asian-Australas J Anim Sci.2019,July 1.  WB ;  Pig.  
研究领域 心血管  神经生物学  信号转导  生长因子和激素  脂蛋白  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat,Dog,Pig,Horse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 103/132kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Leptin receptor: 251-350/1165 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Function:
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity).

Subunit:
On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3. Interaction with SOCS3 inhibits LRb signaling.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Isoform E: Secreted (Probable). Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.

Tissue Specificity:
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.

Post-translational modifications:
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.

Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain.

SWISS:
P48357

Gene ID:
3953

Database links:

Entrez Gene: 3953 Human

Entrez Gene: 16847 Mouse

Entrez Gene: 24536 Rat

Omim: 601007 Human

SwissProt: P48357 Human

SwissProt: P48356 Mouse

SwissProt: Q62959 Rat

Unigene: 23581 Human

Unigene: 723178 Human

Unigene: 259282 Mouse

Unigene: 9891 Rat



Leptin是一种分子量为16kDa的脂肪组织源激素,由脂肪细胞分泌的,具有强亲水性,以单体形式存在于血浆中。 Leptin 具有广泛的生物学效应,作用于下丘脑,调节食欲、能量代谢及体重。Leptin 还可能作为脂肪-胰岛内分泌轴一部分,参与胰岛素分泌的调节。Leptin receptor (OB-R)是与gp130最密切相关的信号转导的共同成分,属1类细胞因子超家族,下丘脑有丰富的Leptin receptor,在其它部位也有分布,但水平较低:如胰岛素B细胞;脉络丛;肝、肺、心、肾、睾丸、淋巴细胞和脂肪细胞中。 人类存在四个异性体,均为单跨膜受体。其中OB-Rb在胞内结构域最长,是唯一能进行信号传导并调节热量摄入和能量消耗的异性体,分子量为132kDa。
产品图片
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103/132 kD
Observed band size: 132 kD
Sample:
Huvec(Human) Cell Lysate at 30 ug
Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103/132 kD
Observed band size: 132 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103/132 kD
Observed band size: 132 kD
Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103/132 kD
Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leptin receptor) Polyclonal Antibody, Unconjugated (bs-20498R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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