| 产品编号 | bs-8747R |
| 英文名称 | Phospholipase D2 Rabbit pAb |
| 中文名称 | 胆碱磷酸酯酶2抗体 |
| 别 名 | Choline phosphatase 2; EC 3.1.4.4; hPLD 2; hPLD2; mPLD2; Phosphatidylcholine hydrolyzing phospholipase D2; Phosphatidylcholine-hydrolyzing phospholipase D2; phospholipase D2; phospholipaseD2; PLD 2; PLD1C; Pld2; PLD2_HUMAN; Pldc; rPLD2. |
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Specific References (1) | bs-8747R has been referenced in 1 publications.
[IF=3.647] Chang Y et al. The in vitro effects of phospholipase D1-mTOR axis in liver fibrogenesis. Life Sci
. 2020 Jun 15;251:117595. IHC ; mouse.
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| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 激酶和磷酸酶 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 106 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLD2/Phospholipase D2: 21-120/933 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospholipase D2) Polyclonal Antibody, Unconjugated (bs-8747R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
