| 产品编号 | bs-20594R |
| 英文名称 | TLR4 |
| 中文名称 | Toll样受体4（CD284）抗体 |
| 别 名 | TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll (Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN. |
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Specific References (43) | bs-20594R has been referenced in 43 publications.
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| 研究领域 | 心血管 免疫学 信号转导 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat,Cow (predicted: Pig,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:500-1000, IF=1:200-1000, ELISA=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 90kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TLR4: 701-800/839 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Post-translational modifications: N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. DISEASE: Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Similarity: Belongs to the Toll-like receptor family. Contains 18 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 TIR domain SWISS: O00206 Gene ID: 7099 Database links: Entrez Gene: 7099 Human Entrez Gene: 21898 Mouse SwissProt: O00206 Human SwissProt: Q9QUK6 Mouse Unigene: 174312 Human Unigene: 38049 Mouse |
| 产品图片 |
Sample:
K562(Human) Cell Lysate at 40 ug HL-60(Human) Cell Lysate at 40 ug RAW264.7(Mouse) Cell Lysate at 40 ug Primary:Anti-TLR4 (bs-20594R) at 1/2000 dilution 
Sample:
HL60 (Human) Lysate at 30 ug Primary: Anti- TLR4 (bs-20594R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 90 kD 
Sample:
HL-60(Human) Cell Lysate at 40 ug U-2OS(Human) Cell Lysate at 40 ug RAW264.7(Mouse) Cell Lysate at 40 ug BV2(Mouse) Cell Lysate at 40 ug DU145(Human) Cell Lysate at 40 ug K562(Human) Cell Lysate at 40 ug Embryo (Mouse) Lysate at 40 ug Primary: Anti-TLR4 (bs-20594R) at 1/300 dilution 
Sample:
Lane 1: Jurkat (Human) Cell Lysate at 30 ug Lane 2: HUVEC (Human) Cell Lysate at 30 ug Lane 3: U-2OS (Human) Cell Lysate at 30 ug Lane 4: Lymph node (Mouse) Lysate at 40 ug Lane 5: Raw264.7 (Mouse) Cell Lysate at 30 ug Primary: Anti-TLR4 (bs-20594R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100-135 kD Observed band size: 120 kD 
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: Lymph node (Rat) Lysate at 40 ug Lane 3: BV2-lps (Mouse) Cell Lysate at 30 ug Lane 4: HL60 (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Lane 6: A549 (Human) Cell Lysate at 30 ug Primary: Anti-TLR4 (bs-20594R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 135 kD 
This image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- TLR4 Polyclonal Antibody (bs-20594R) at 1:300 for 3 hours at 4°C, followed by Alexa Fluor 488-conjugated secondary antibody for an additional hour.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
