[IF=38.104] Chen, Lei. et al. SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl− accumulation in respiratory epithelium. SIGNAL TRANSDUCT TAR. 2022 Jul;7(1):1-13  IF ;  Human.  

PubMed:35896532

[IF=17.694] Wang Kaiyuan. et al. Gas therapy potentiates aggregation-induced emission luminogen-based photoimmunotherapy of poorly immunogenic tumors through cGAS-STING pathway activation. NAT COMMUN. 2023 May;14(1):1-19  WB ;  Mouse.  

PubMed:37221157

[IF=3.375] Zhen Zhang. et al. Tanshinone IIA regulates fibroblast proliferation and migration and post-surgery arthrofibrosis through the autophagy-mediated PI3K and AMPK-mTOR signaling pathway. Am J Transl Res. 2021; 13(2): 565–584  IHC ;  Rabbit.  

PubMed:33594310

[IF=2.794] Shi, Linjie. et al. Memory regulation in feeding habit transformation to dead prey fish of Chinese perch (Siniperca chuatsi). 2021 Sep 28  WB ;  Chinese perch.  

PubMed:34581919

[IF=2.2] Li, Jiao. et al. Adaptation of AMPK-mTOR-signal pathways and lipid metabolism in response to low- and high-level rapeseed meal diet in Chinese perch (Siniperca chuatsi). J Comp Physiol B. 2021 Sep;191(5):881-894  WB ;  Fish.  

PubMed:34373935

[IF=1.564] Wang Yuan. et al. Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses. ACTA CIR BRAS. 2022 Dec;37:  WB ;  Rat.  

PubMed:36515323

Sample:
U-87 MG (Human) Lysate at 30 ug
Primary: Anti- MMP-2 (bs-20705R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
U937 (Human) Lysate at 30 ug
Primary: Anti- MMP-2 (bs-20705R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
Cerebellum (Mouse) Lysate at 40 ug
Cerebellum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-20694R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Polyclonal Antibody, Unconjugated (bs-20694R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control (blue line): Hela(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (bs-20694R), Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (bs-20694R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (bs-20694R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.