| 产品编号 | bs-23149R |
| 英文名称 | PACAP receptor |
| 中文名称 | 腺苷酸环化酶激活肽受体1抗体 |
| 别 名 | ADCYAP1 R1; ADCYAP1R 1; PACAP-R1; adenylate cyclase activating polypeptide 1 (pituitary) receptor type 1; PAC 1; PAC1; PAC1R; PACAP R 1; PACAP1 R; PACAPR; PACAPRI; Pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; Pituitary adenylate cyclase activating polypeptide receptor; Pituitary adenylate cyclase activating polypeptide type I receptor precursor; PACR_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 细胞膜受体 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse,Human (predicted: Sheep,Rabbit,Horse,Cow,Pig,Dog) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACAP receptor: 151-250/468 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide belonging to the vasoactive intestinal polypeptide/glucagon/ secretin family. It is widely distributed in the body, and a variety of biological actions have been reported. Recent studies have shown that there is a family of PACAP receptors (PACAPRs), and two members of this family have been identified. This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract (By similarity). Belongs to the G-protein coupled receptor 2 family. Function: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Subunit: Interacts (via N-terminal extracellular domain) with ADCYAP1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta. Similarity: Belongs to the G-protein coupled receptor 2 family. SWISS: P41586 Gene ID: 117 Database links: Entrez Gene: 117 Human Entrez Gene: 11517 Mouse Omim: 102981 Human SwissProt: P41586 Human SwissProt: P70205 Mouse Unigene: 377783 Human Unigene: 44245 Mouse Unigene: 88408 Rat 生长因子和激素（ Growth Factor and Hormones） PACAP 受体分为两个亚型，PACAP-1型受体对PACAP是特异性的，与PACAP的亲和力比VIP高100-1000倍，PACAP-II型受体与PACAP和VIP的亲和力相似，对二者无选择性。此抗体即为前者。 |
| 产品图片 |
Sample:
Lane 1: Mouse Heart tissue lysates Lane 2: Rat Placenta tissue lysates Lane 3: Human HeLa cell lysates Lane 4: Human SH-SY5Y cell lysates Lane 5: Human U87MG cell lysates Primary: Anti-PACAP receptor (bs-23149R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 53,60 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
