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Mouse Anti-MBP tag  antibody (bsm-33008M)
~~~促销,代码KX240301~~~
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说明书: 50ul  100ul  500ul
50ul/680.00元
100ul/980.00元
500ul/4000.00元
大包装/询价

产品编号 bsm-33008M
英文名称 MBP tag
中文名称 麦芽糖结合蛋白单克隆抗体
别    名 MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI.  
Specific References  (1)     |     bsm-33008M has been referenced in 1 publications.
[IF=3.197] Wei Lu. et al. Molecular mechanisms by which targeted muscle reinnervation improves the microenvironment of spinal cord motor neurons and target muscles. NEUROSCI LETT. 2022 Oct;789:136879  IHC ;  Rat.  
产品类型 标签抗体 
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 9G2
交叉反应 Recombinant protein
产品应用 WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 44kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant MBP Protein of Escherichia coli (strain K12) 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Function:
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.

SWISS:
P0AEX9

Gene ID:
1039564

Database links:
Entrez Gene: 1039564 Escherichia coli

Entrez Gene: 4494002 Escherichia coli

Entrez Gene: 948538 Escherichia coli

Entrez Gene: 960089 Escherichia coli

SwissProt: P0AEX9 Escherichia coli

SwissProt: P0AEY0 Escherichia coli



产品图片
Sample:
Lane 1: MBP tagged cytochrome C (Full Length) Protein (bs-41232P) at 4 ug
Lane 2: MBP tagged caspase 3 p17 (143-213) Protein (bs-41233P) at 4 ug
Primary: Anti-MBP tag (bsm-33008M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60-70 kD
Observed band size: 62 kD
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