| 产品编号 | bsm-33053M |
| 英文名称 | AMACR Mouse mAb |
| 中文名称 | α-甲基酰基辅酶A消旋酶单克隆抗体 |
| 别 名 | AMACR_HUMAN; alpha-methylacyl-CoA racemase; EC:5.1.99.4; 2-methylacyl-CoA racemase; RM; RACE; CBAS4; P504S; AMACRD; |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 新陈代谢 表观遗传学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 6B3 |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human AMACR protein: full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011] Function: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Subcellular Location: Peroxisome. Mitochondrion. DISEASE: Alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]: A rare autosomal recessive peroxisomal disorder characterized by elevated plasma concentrations of pristanic acid C27-bile-acid intermediates, and adult onset of variable neurodegenerative symptoms affecting the central and peripheral nervous systems. Features may include seizures, visual failure, sensorimotor neuropathy, spasticity, migraine, and white matter hyperintensities on brain imaging. Note=The disease is caused by mutations affecting the gene represented in this entry. Congenital bile acid synthesis defect 4 (CBAS4) [MIM:214950]: A disorder characterized by the presence of trihydroxycoprostanic acid in the bile and absence of cholic acid. Patients manifest neonatal jaundice, intrahepatic cholestasis and bile duct deficiency. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the CaiB/BaiF CoA-transferase family. SWISS: Q9UHK6 Gene ID: 23600 Database links: Entrez Gene: 23600 Human Omim: 604489 Human SwissProt: Q9UHK6 Human Unigene: 508343 Human AMACR的优点在于它是癌症特异性，在癌症组织中高表达。 AMACR亦可用作其他癌症的诊断标志物。对各种癌症细胞进行检查后发现，结肠直肠癌、卵巢癌、乳腺癌、膀胱癌、肺癌、淋巴瘤和黑素瘤都过度表达AMACR，以结肠直肠癌和前列腺癌表达最高。 AMACR是一种新型前列腺癌标记物，在前列腺癌中胞浆表达较多，正常表到较少. |
| 产品图片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Liver tissue lysates
Primary: Anti- AMACR (bsm-33053M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- AMACR (bsm-33053M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
