| 产品编号 | bsm-33185M |
| 英文名称 | EBFP Mouse mAb |
| 中文名称 | 增强型蓝色荧光蛋白单克隆抗体 |
| 别 名 | Blue Fluorescent Protein; Enhanced Blue Fluorescent Protein. 蓝色荧光蛋白的工程变体蛋白 |
| 产品类型 | 标签抗体 |
| 研究领域 | 细胞生物 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 7B12 |
| 交叉反应 | Species independent |
| 产品应用 | WB=1:1000-5000,ELISA=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant EBFP protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. |
| 产品图片 |
Sample:
Lane 1: EBFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33185P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- EBFP-Tag (bsm-33185M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
