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PPAR alpha Rabbit pAb (bs-23398R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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产品编号 bs-23398R
英文名称 PPAR alpha Rabbit pAb
中文名称 α型-过氧化酶活化增生受体抗体
别    名 hPPAR; MGC2237; MGC2452; NR1C1; Nuclear receptor subfamily 1 group C member 1; Peroxisome Proliferator Activated Receptor alpha; PPAR; PPARA; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; PPARA_HUMAN; PPARalpha.  PPAR α; PPAR-α; PPARα;
Specific References  (17)     |     bs-23398R has been referenced in 17 publications.
[IF=9.988] Hao Ni. et al. Long term toxicities following developmental exposure to perfluorooctanoic acid: Roles of peroxisome proliferation activated receptor alpha. ENVIRON POLLUT. 2023 Jan;317:120722  WB ;  Chicken.  
[IF=8.071] Xiaohui Xu. et al. Hexafluoropropylene oxide dimer acid (HFPO-DA) induced developmental cardiotoxicity and hepatotoxicity in hatchling chickens: Roles of peroxisome proliferator activated receptor alpha. Environ Pollut. 2021 Dec;290:118112  WB ;  chicken.  
[IF=7.129] Qixuan Dong. et al. Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved. ECOTOX ENVIRON SAFE. 2023 Mar;253:114671  WB ;  Chicken.  
[IF=6.208] Yongzhi Sun. et al. GB1a Activates SIRT6 to Regulate Lipid Metabolism in Mouse Primary Hepatocytes. INT J MOL SCI. 2023 Jan;24(11):9540  WB ;  Human.  
[IF=6.025] Yue-Qiang Huang. et al. Di-2-ethylhexyl phthalate (DEHP) induced lipid metabolism disorder in liver via activating the LXR/SREBP-1c/PPARα/γ and NF-κB signaling pathway. FOOD CHEM TOXICOL. 2022 Jul;165:113119  WB ;  Bird.  
[IF=5.742] Pan Cao. et al. Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1α. WORLD J GASTROENTERO. 2022 May 7; 28(17): 1798–1813  WB ;  Mouse,Human.  
[IF=4.96] L.X. Li. et al. MiR-23a-5p exacerbates intestinal ischemia–reperfusion injury by promoting oxidative stress via targeting PPAR alpha. Biochem Pharmacol. 2020 Oct;180:114194  WB ;  Rat.  
[IF=4.784] Pan Y et al. Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Int J Biol Macromol. 2019 Nov 8. pii: S0141-8130(19)37563-4.  WB ;  Rat.  
[IF=4.533] Huiying Gu. et al. Soluble Klotho Improves Hepatic Glucose and Lipid Homeostasis in Type 2 Diabetes. Mol Ther-Meth Clin D. 2020 Sep;18:811  WB ;  Mouse.  
[IF=4.432] Pan Cao. et al. Pinocembrin ameliorates acute liver failure via activating the Sirt1/PPARα pathway in vitro and in vivo. Eur J Pharmacol. 2022 Jan;915:174610  WB ;  Mouse.  
[IF=4.398] Xiaoyu Qu. et al. Integration of metabolomics and proteomics analysis to explore the mechanism of neurotoxicity induced by receipt of isoniazid and rifampicin in mice. NEUROTOXICOLOGY. 2023 Jan;94:24  IHC ;  Mouse.  
[IF=4.358] Jin X et al. Fine particles cause the abnormality of cardiac ATP levels via PPARɑ-mediated utilization of fattyacid and glucose using in vivo and in vitro models.Environ Pollut. 2019 Jun;249:286-294.  WB/IF ;  Rat.  
[IF=3.234] Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29.  WB ;  Mouse.  
[IF=3.188] Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.  IHF ;  Rat.  
[IF=2.629] Zang Zhen-Zhong. et al. Uncovering the Protective Mechanism of the Volatile Oil of Acorus tatarinowii against Acute Myocardial Ischemia Injury Using Network Pharmacology and Experimental Validation. Evid-Based Compl Alt. 2021;2021:6630795  WB ;  Mouse.  
[IF=2.534] Dongmin Zou. et al. BHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153  WB ;  Sheep.  
[IF=1.851] Liu Q et al. Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2. Molecular Medicine Reports.2019,19: 5087-5096 .  WB ;  Rat&Mouse.  
研究领域 肿瘤  免疫学  信号转导  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Chicken,Dog,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 51 kDa
检测分子量
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PPAR alpha: 181-280/468 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

Function:
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

Subunit:
Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its transcriptional activity. Interacts with NCOA3 and NCOA6 coactivators. Interacts with ASXL1 AND ASXL2.

Subcellular Location:
Nucleus.

Tissue Specificity:
Skeletal muscle, liver, heart and kidney.

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
Q6I9S0

Gene ID:
5465

Database links:

Entrez Gene: 5465 Human

Entrez Gene: 19013 Mouse

Entrez Gene: 25747 Rat

Omim: 170998 Human

SwissProt: Q07869 Human

SwissProt: Q6I9S0 Human

SwissProt: P23204 Mouse

SwissProt: Q542P9 Mouse

SwissProt: P37230 Rat

Unigene: 103110 Human

Unigene: 710044 Human

Unigene: 212789 Mouse

Unigene: 9753 Rat



产品图片
Sample: Heart(Mouse) Lysate at 40 ug Primary: Anti- PPAR alpha (bs-7114R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PPAR alpha (bs-23398R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PPAR gamma 2 Polyclonal Antibody, Unconjugated(bs-7114R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PPAR alpha antibody (bs-23398R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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