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Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R)
~~~促销,代码KT2401~~~
订购热线:400-901-9800
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说明书: 50ul  100ul  500ul
50ul/680.00元
100ul/980.00元
500ul/4000.00元
大包装/询价

产品编号 bs-0061R
英文名称 beta-Actin (Loading Control)
中文名称 β-肌动蛋白/β-Actin(内参)抗体
别    名 Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin; A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.  β actin; βactin;
Specific References  (1140)     |     bs-0061R has been referenced in 1140 publications.
[IF=3.192]   WB ;  Pig.  
[IF=4.087]   WB ;  Rat.  
[IF=20.042] Xiang-Qian Gao. et al. The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N 6 -methyladenosine methylation of Parp10 mRNA. Nat Cell Biol. 2020 Oct;22(11):1319-1331  WB ;  Mouse.  
[IF=19.227] Xin Zhou. et al. ACSL4 promotes microglia-mediated neuroinflammation by regulating lipid metabolism and VGLL4 expression. BRAIN BEHAV IMMUN. 2023 Mar;109:331  WB ;  Mouse.  
[IF=18.027] Nguyen Thi Nguyen. et al. Amplified Fenton-Based Oxidative Stress Utilizing Ultraviolet Upconversion Luminescence-Fueled Nanoreactors for Apoptosis-Strengthened Ferroptosis Anticancer Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX  WB ;  Mouse.  
[IF=16.744] Rui Liu. et al. Engineered stem cell biomimetic liposomes carrying levamisole for macrophage immunity reconstruction in leukemia therapy. CHEM ENG J. 2022 Nov;447:137582  WB ;  Rat.  
[IF=15.84] Xiaolie He. et al. MgFe‐LDH Nanoparticles: A Promising Leukemia Inhibitory Factor Replacement for Self‐Renewal and Pluripotency Maintenance in Cultured Mouse Embryonic Stem Cells. 2021 Feb 25  WB ;  Mouse.  
[IF=15.304] Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635  WB ;  Mouse.  
[IF=14.976] Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer‐induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068  WB ;  Mouse.  
[IF=14.903] Shuai Xiong. et al. Glutamate-releasing BEST1 channel is a new target for neuroprotection against ischemic stroke with wide time window. ACTA PHARM SIN B. 2023 May;:  WB ;  Mouse.  
[IF=14.26] Mi Bai. et al. LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease. EMBO MOL MED. 2023 Jan 11  WB ;  Mouse.  
[IF=14.026] Congcong Chen. et al. Radix Paeoniae Alba attenuates Radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase. J PHARM ANAL. 2023 Apr;:  WB ;  Rat.  
[IF=13.273] Danmin Lin. et al. Brain-targeted gene delivery of ZnO quantum dots nanoplatform for the treatment of Parkinson disease. Chem Eng J. 2022 Feb;429:132210  WB ;  Human.  
[IF=13.273] Long Zhao. et al. Juglone-loaded metal-organic frameworks for H2O2 self-modulating enhancing chemodynamic therapy against prostate cancer. Chem Eng J. 2022 Feb;430:133057  WB ;  Human.  
[IF=13.273] Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094  WB ;  Mouse.  
[IF=12.336] Baikai Ma. et al. Pigment epithelium-derived factor (PEDF) plays anti-inflammatory roles in the pathogenesis of dry eye disease. Ocul Surf. 2021 Apr;20:70  WB ;  Human.  
[IF=11.556] Wen-juan Jiang. et al. Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics. 2022; 12(1): 324–339  WB ;  Mouse,Human.  
[IF=11.48] Lan, Feng, et al. "Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3." Journal of Allergy and Clinical Immunology 132.6 (2013): 1314-1321.  WB ;  Human.  
[IF=11.33] Leclercq, Sophie, et al. "Low-dose penicillin in early life induces long-term changes in murine gut microbiota, brain cytokines and behavior." Nature Communications 8 (2017): 15062.  WB ;  Mouse.  
[IF=11.161] Zhipeng Jiang. et al. EIF4A3-induced circ_0084615 contributes to the progression of colorectal cancer via miR-599/ONECUT2 pathway. J Exp Clin Canc Res. 2021 Dec;40(1):1-15  WB ;  Human.  
[IF=10.787] Xiaozhen Dai. et al. Nrf2 transcriptional upregulation of IDH2 to tune mitochondrial dynamics and rescue angiogenic function of diabetic EPCs. REDOX BIOL. 2022 Oct;56:102449  WB ;  Mouse.  
[IF=10.787] Yi Zhao. et al. Ferroptosis is critical for phthalates driving the blood-testis barrier dysfunction via targeting transferrin receptor. REDOX BIOL. 2023 Feb;59:102584  WB ;  Mouse.  
[IF=10.753] Fangwei Yang. et al. Diethyl phosphate disrupts hypothalamus-pituitary-adrenal axis endocrine hormones via nuclear receptors GR and Nur77: Integration of evidences from in vivo, in vitro and in silico approaches. SCI TOTAL ENVIRON. 2022 Oct;844:157015  WB ;  Rat, Human.  
[IF=10.753] Ya-Nan Gao. et al. Aflatoxin M1 and ochratoxin A induce a competitive endogenous RNA regulatory network of intestinal immunosuppression by whole-transcriptome analysis. SCI TOTAL ENVIRON. 2022 Sep;:158777  WB ;  Human.  
[IF=10.75] Hu Yifang. et al. S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development. INT J BIOL SCI. 2023 Apr;19(7):2034-2052  WB ;  Human.  
[IF=10.383] Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse,Human.  
[IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119  WB ;  Mouse.  
[IF=10.171] Ping Pang. et al. The circular RNA circHelz enhances cardiac fibrosis by facilitating the nuclear translocation of YAP1. TRANSL RES. 2023 Feb;:  WB ;  Mouse.  
[IF=9.933] Xingyi Xu. et al. A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy. 2021 Jul 23  WB ;  Human.  
[IF=9.918] Jie Hao. et al. Multifunctional miR181a nanoparticles promote highly efficient radiotherapy for rectal cancer. MATER TODAY ADV. 2022 Dec;16:100317  WB ;  Mouse.  
产品类型 内参抗体 
研究领域 肿瘤  细胞生物  信号转导  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Hamster, 3 (predicted: Chicken, Dog, Pig, Rabbit, Sheep, Bee, Fish, Guinea Pig, Cat, 2)
产品应用 WB=1:5000-50000 ELISA=1:5000-20000 IHC-P=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 42kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Synthetic MAP peptide derived from human beta-Actin: 1-200/375 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Loading Control
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].

Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2.

Subcellular Location:
Cytoplasm. cytoskeleton.

Tissue Specificity:
Ubiquitously expressed in all eukaryotic cells.

Post-translational modifications:
ISGylated.
Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

DISEASE:
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.

Similarity:
Belongs to the actin family.

SWISS:
P60709

Gene ID:
60

Database links:

Entrez Gene: 396526 Chicken

Entrez Gene: 60 Human

Entrez Gene: 11461 Mouse

Entrez Gene: 100009272 Rabbit

Entrez Gene: 81822 Rat

Omim: 102630 Human

SwissProt: P60706 Chicken

SwissProt: P60712 Cow

SwissProt: P60708 Horse

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P29751 Rabbit

SwissProt: P60711 Rat

SwissProt: P60713 Sheep

Unigene: 520640 Human

Unigene: 708120 Human

Unigene: 727576 Human

Unigene: 328431 Mouse

Unigene: 391967 Mouse

Unigene: 94978 Rat



内参抗体
β-Actin是横纹肌肌纤维中的一种主要蛋白质成分,也是肌肉细丝及细胞骨架微丝的主要成分。具有收缩功能,分布广泛,具有高度保守性,在细胞中的表达相对稳定,因此常被用作校正系统的内参。β-Actin分子量为42 kDa,
此抗体主要用于标记平滑肌及其来源的肿瘤。
我公司开发的β-Actin抗体已被国内外广大科研工作者使用,被称为质量信得过产品.
产品图片
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lane1: 293T Cell Lysate at 25 ug
Lane2: A549 Cell Lysate at 25 ug
Lane3: A431 Cell Lysate at 25 ug
Primary: Anti- beta-Actin (bs-0061R) at 1/1000 and 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42 kD
Sample: Lung lysate at 30ug;
Primary: Anti-beta-actin (bs-0061R) at 1:1000 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1:3000 dilution
Predicted band size : 42kD
Observed band size : 42kD
Sample:
SH-SY5Y (Human) Lysate at 40 ug
Primary:
Anti-beta-Actin (bs-0061R) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary:
Anti-beta-Actin (bs-0061R) at 1/1000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
HL60 (Human) Cell Lysate at 40 ug
HUVEC (Human) Cell Lysate at 40 ug
Spinal cord (Rat) Lysate at 40 ug
Rectum (Rat) Lysate at 40 ug
Placenta (Rat) Lysate at 40 ug
Lymph node (Rat) Lysate at 40 ug
Lung (Rat) Lysate at 40 ug
Spleen (Rat) Lysate at 40 ug
JAR (Human) Cell Lysate at 40 ug
293T (Human) Cell Lysate at 40 ug
Jurkat (Human) Cell Lysate at 40 ug
TM4 (Human) Cell Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
A549 (Human) Cell Lysate at 40 ug
Raw264.7 (Mouse) Cell Lysate at 40 ug
SH-SY5Y (Human) Cell Lysate at 40 ug
MKN45 (Human) Cell Lysate at 40 ug
CHO (Hamster) Cell Lysate at 40 ug
Panc-1 (Human) Cell Lysate at 40 ug
4T1 (Mouse) Cell Lysate at 40 ug
ASPC-1 (Human) Cell Lysate at 40 ug
H9C2 (Rat) Cell Lysate at 40 ug
Brl-3a (Rat) Cell Lysate at 40 ug
TT (Human) Cell Lysate at 40 ug
TEV-1 (Human) Cell Lysate at 40 ug
EC9706 (Human) Cell Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Urinary bladder (Mouse) Lysate at 40 ug
Uterus (Mouse) Cell Lysate at 40 ug
Aorta (Mouse) Lysate at 40 ug
olfactory bulb (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Adrenal gland (Mouse) Lysate at 40 ug
Ovary (Mouse) Lysate at 40 ug
Ear (Mouse) Lysate at 40 ug
U2os (Human) Cell Lysate at 40 ug
ASPC-1 (Human) Cell Lysate at 40 ug
Vas deferens (Mouse) Lysate at 40 ug
trachea (Mouse) Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Embryo Cerebrum (Mouse) Lysate at 40 ug
Du145 (Human) Lysate at 40 ug
SW480 (Human) Cell Lysate at 40 ug
U87MG (Human) Lysate at 40 ug
U251 (Human) Lysate at 40 ug
A673 (Human) Lysate at 40 ug
Lovo (Human) Lysate at 40 ug
293FT (Human) Lysate at 40 ug
JEG-3 (Human) Lysate at 40 ug
RSC96 (Rat) Cell Lysate at 40 ug
MCF-7 (Human) Cell Lysate at 40 ug
HepG2 (Human) Lysate at 40 ug
A431 (Human) Lysate at 40 ug
Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-beta-Actin (Loading Control) (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
Small intestine (Mouse) Lysate at 40 ug
Primary: Anti- beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated(bs-0061R) 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: NIH/3T3.
Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: RSC96(blue).
Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody (bs-0061R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-0061R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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