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RIPA Lysis Buffer (C05-01001)
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说明书: 100ml  
100ml/200.00元
大包装/询价
产品编号 C05-01001
英文名称 RIPA Lysis Buffer
中文名称 RIPA裂解液(W)/RIPA裂解液(弱)
别    名 Radio Immunoprecipitation Assay Lysis Buffer; RIPA Lysis Buffer, Medium; RIPA Lysis Buffer (Medium); Medium; RIPA Lysis Buffer (M); Medium RIPA Lysis Buffer; Mammalian Total Protein Extraction Kit; Protein Extraction Kit;  细胞裂解液; 组织细胞裂解液; 蛋白提取液; RIPA裂解液(弱);
Specific References  (19)     |     C05-01001 has been referenced in 19 publications.
[IF=6.638] Wang Z et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guidedby drug-mediated computed tomography imaging. Acta Biomater. 2019 May 22.  Other ;  
[IF=6.064] Tianyu Zhang. et al. Different Extracellular β-Amyloid (1-42) Aggregates Differentially Impair Neural Cell Adhesion and Neurite Outgrowth through Differential Induction of Scaffold Palladin. BIOMOLECULES. 2022 Dec;12(12):1808  
[IF=5.878] Hong-He Xiao. et al. Icarisid II rescues cognitive dysfunction via activation of Wnt/β-catenin signaling pathway promoting hippocampal neurogenesis in APP/PS1 transgenic mice. 2022 Mar 01  Other ;  Other.  
[IF=5.572] Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724  
[IF=5.443] Li Wenjing. et al. CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes. Stem Cells Int. 2021;2021:6644366  WB ;  Human.  
[IF=4.757] Yunna Zhang. et al. Two-Pore-Domain Potassium Channel TREK–1 Mediates Pulmonary Fibrosis through Macrophage M2 Polarization and by Direct Promotion of Fibroblast Differentiation. BIOMEDICINES. 2023 May;11(5):1279  
[IF=3.212] Shang-Ping Zhao. et al. Long Non-coding RNA FENDRR Modulates Autophagy Through Epigenetic Suppression of ATG7 via Binding PRC2 in Acute Pancreatitis. 2021 Jan 08  
[IF=2.985] Tao Song. et al. FLOT2 upregulation promotes growth and invasion by interacting and stabilizing EphA2 in gliomas. Biochem Bioph Res Co. 2021 Apr;548:67  other ;  
[IF=2.967] An Liu. et al. MiRNA-3662 reverses the gemcitabine resistance in pancreatic cancer through regulating the tumor metabolism. 2021 May 15  Other ;  
[IF=2.737] A. Liu. et al. Circ_03955 promotes pancreatic cancer tumorigenesis and Warburg effect by targeting the miR-3662/HIF-1α axis. 2021 Apr 17  Other ;  
[IF=2.311] Yang H et al. Long non-coding RNA EWSAT1 contributes to the proliferation and invasion of glioma by sponging miR‑152‑3p. Oncol Lett. 2020 Aug;20(2):1846-1854.  WB ;  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
[IF=1.902] Xiong Qian. et al. Tectorigenin regulates migration, invasion, and apoptosis in dexamethasone‐induced human airway epithelial cells through up‐regulating miR‐222‐3p. 2021 Feb 04  other ;  
[IF=1.894] Qian D et al. Roles of CCNB2 and NKX3-1 in Nasopharyngeal Carcinoma. Cancer Biother Radiopharm. 2020 Apr;35(3):208-213.  WB ;  human.  
[IF=1.871] Bi H et al. Interleukin‑8 promotes cell migration via CXCR1 and CXCR2 in liver cancer. ONCOLOGY LETTERS.2019 18: 4176-4184.  WB ;  
[IF=1.785] Hui Yang. et al. Upregulated LINC00319 aggravates neuronal injury induced by oxygen‑glucose deprivation via modulating miR‑200a‑3p. Exp Ther Med. 2021 Aug;22(2):1-10  Other ;  
[IF=1.785] An J et al. A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen‑thawed embryo transfer. Exp Ther Med . 2020 Sep;20(3):2191-2199.  WB ;  
[IF=1.777] Wenbo Zhao. et al. The effect of the WKYMVm peptide on promoting mBMSC secretion of exosomes to induce M2 macrophage polarization through the FPR2 pathway. J Orthop Surg Res. 2021 Dec;16(1):1-14  Other ;  
[IF=1.492] Xu S et al. Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/β-catenin pathway.(2018) Braz. J. Med. Biol. Res.;52(1)  WB ;  
保存条件 RIPA裂解液于2-8℃可保存一年;PMSF置于-20 ℃保存。
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

简介:<br />
RIPA裂解液(RIPA Lysis Buffer)是一种传统的细胞组织快速裂解液。RIPA裂解液裂解得到的蛋白样品可以用于常规的Western、IP等。<br />
RIPA裂解液的主要成分为主要成分为50mM Tris(pH 7.4),150mM NaCl,1% NP-40,0.5% sodium deoxycholate,0.1% SDS、EDTA等,另加有蛋白酶抑制剂Aprotinin等可以有效抑制蛋白降解。<br />
<br />
使用说明:<br />
根据使用量,取每1ml RIPA 加入10ul PMSF,使PMSF的最终浓度为1mM。混匀备用(PMSF现用现加)。<br />
1、样品前处理:<br />
a)对于贴壁细胞:去除培养液,用 PBS、生理盐水或无血清培养液洗一遍。按照 6 孔板每孔细胞量加入150-250 ul裂解液的比例加入裂解液。用枪吹打数下,使裂解液和细胞充分接触。<br />
b)对于悬浮细胞:离心收集细胞,用手指把细胞用力弹散。按照6孔板每孔细胞量加入150-250ul裂解液的比例加入裂解液,再用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多,必需分装成50-100万细胞/管,然后再裂解。<br />
c)对于组织样品: 把组织剪切成细小的碎片。按照每20mg组织加入150-250 ul裂解液的比例加入裂解液。 (如果裂解不充分可以适当添加更多的裂解液,如果需要高浓度的蛋白样品,可以适当减少裂解液的用量)。<br />
用玻璃匀浆器匀浆,直至充分裂解。<br />
2、后处理:<br />
将裂解后的样品10000-14000g离心3-5分钟,取上清,即可进行后续的PAGE、Western和免疫沉淀等操作。<br />
<br />
注意事项:<br />
1、 因SDS在低温易析出,如发现RIPA有沉淀,请先使其恢复室温,沉淀即可溶解。<br />
2、 裂解蛋白的所有步骤都需在冰上或4℃进行。<br />

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