| 产品编号 | bs-23779R |
| 英文名称 | CPT1A |
| 中文名称 | 肉毒碱棕榈酰基转移酶1A抗体 |
| 别 名 | CPT 1; CPT1A; CPTI-L; CPT1-L; carnitine palmitoyl transferase 1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT I; Carnitine palmitoyltransferase 1A; carnitine O-palmitoyltransferase 1, liver isoform isoform 1; CPT1A_HUMAN. |
 |
Specific References (6) | bs-23779R has been referenced in 6 publications.
[IF=8.886] Bolin Cai. et al. LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with sarcoplasmic/endoplasmic reticulum calcium ATPase 2. Mol Ther-Nucl Acids. 2021 Dec;: WB ; Chicken.
[IF=8.469] Cai, Bolin. et al. Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development. CELL DEATH DIS. 2022 Apr;13(4):1-12 WB ; Chicken.
[IF=7.525] Ma Manting. et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-16 WB ; Chicken.
[IF=2.72] Tiange Li. et al. Chitosan oligosaccharide attenuates hepatic steatosis in HepG2 cells via the activation of AMP-activated protein kinase. 2022 Feb 20 WB ; Human.
[IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292 WB ; Rat.
[IF=1.832] Yao C et al. Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs. Animals (Basel). 2019 Apr 1;9(4). pii: E137. WB ; Pig.
|
| 研究领域 | 肿瘤 免疫学 信号转导 激酶和磷酸酶 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Chicken,Dog,Pig,Cow) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 86kDa |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CPT1A: 351-450/773 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The mitochondrial oxidation of long-chain fatty acids is nitiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism. Subunit: Homohexamer and homotrimer. Identified in a complex that contains at least CPT1A, ACSL1 and VDAC1. Also identified in complexes with ACSL1 and VDAC2 and VDAC3. Subcellular Location: Mitochondrion outer membrane; Multi-pass membrane protein. Tissue Specificity: Strong expression in kidney and heart, and lower in liver and skeletal muscle. DISEASE: Carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]: Rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the carnitine/choline acetyltransferase family. SWISS: P50416 Gene ID: 1374 Database links: Entrez Gene: 1374 Human Entrez Gene: 12894 Mouse Omim: 600528 Human SwissProt: P50416 Human SwissProt: P97742 Mouse Unigene: 503043 Human Unigene: 18522 Mouse Unigene: 2856 Rat
|
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CPT1A (bs-23779R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD 
Sample:
Cerebrum (Rat) Lysate at 40 ug Primary: Anti-CPT1A (bs-23779R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD 
Sample:
Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-CPT1A (bs-23779R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD 
Sample:
Kidney (Mouse) Lysate at 40 ug Primary: Anti- CPT1A (bs-23779R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
