客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 常用试剂 > 产品信息
Annexin V-FITC Apoptosis Detection Kit (BA00101)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:support@bioss.com.cn
说明书: 下载
20T/600.00元
50T/1180.00元
100T/2100.00元
大包装/询价

产品编号 BA00101
英文名称 Annexin V-FITC Apoptosis Detection Kit
中文名称 Annexin V-FITC细胞凋亡检测试剂盒
别    名 Annexin V-FITC/PI Apoptosis Detection Kit; Annexin V FITC Apoptosis Detection Kit; Annexin V-FITC/PI Kit; FITC Annexin V Apoptosis Detection Kit I;  
Specific References  (2)     |     BA00101 has been referenced in 2 publications.
[IF=3.432] Dong W et al. Polychlorinated biphenyl quinone induces caspase 1-mediated pyroptosis through the induction of pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD signal axis. Chem Res Toxicol. 2019 Apr 23.   Other ;  Human.  
[IF=3.547] Li B et al. Role of c-Myc/chloride intracellular channel 4 pathway in lipopolysaccharide-induced neurodegenerative diseases. Toxicology. 2019 Nov 3;429:152312.   cell apoptosis ;  
保存条件 Store at 4℃.
产品介绍 磷脂酰丝氨酸(PS)是一种带负电荷的磷脂,正常细胞中,PS只分布在细胞膜脂质双层的内侧,而在细胞凋亡早期,细胞膜 PS由脂膜内侧翻向细胞膜外侧,使PS暴露在细胞膜外表面。Annexin V是一种分子量为35~36kD 的Ca2+依赖性磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。因此Annexin V被公认为检测细胞早期凋亡的灵敏指标之一。
将Annexin V进行FITC标记,以标记了的Annexin V作为探针,利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。碘化丙啶(Propidium Iodide, PI)是一种核酸染料,它不能透过正常细胞或早期凋亡细胞的完整的细胞膜,但对凋亡中晚期的细胞和坏死细胞,PI能够透过细胞膜而使细胞核染红。因此采用Annexin V与PI双染的方法,就可以将处于不同凋亡时期的细胞区分开来。
产品图片
Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. Propidium Iodide (PI) is a standard flow cytometric viability probe and is used to distinguish viable from nonviable cells. Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. Cells that stain positive for FITC Annexin V and negative for PI are undergoing apoptosis. Cells that stain positive for both FITC Annexin V and PI are either in the end stage of apoptosis, are undergoing necrosis, or are already dead.
Jurkat cells (T-cell leukemia,human) treated with 10 μM of camptothecin for 4 hours(panel right) or untreated control(panel left).
1. Wash cells twice with cold PBS and then resuspend cells in 1×Binding Buffer at a concentration of 1×106 cells/ml.
2. Transfer 100 μl of the solution (1×105cells) to a 5 ml culture tube.
3. Add 5 μl of Annexin V-FITC.
4. Add 5 μl PI.
5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
6. Add 400 μl of 1×Binding Buffer to each tube.Analyze by flow cytometry within 1 hr.
版权所有 2019-2022 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号