| 产品编号 | bsm-51077M |
| 英文名称 | RAB8A |
| 中文名称 | ras癌基因家族Rab8蛋白单克隆抗体 |
| 别 名 | MEL; Mel transforming oncogene; Oncogene c mel; Oncogene c-mel; RAB8; RAB8A; RAB8A member RAS oncogene family; RAB8A_HUMAN; Ras associated protein RAB8; Ras related protein Rab 8A; Ras-related protein Rab-8A. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 8A4 |
| 交叉反应 | Human (predicted: Mouse) |
| 产品应用 | WB=1:100-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant RAB8A protein |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. Function: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Subunit: Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3 (By similarity). Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Golgi apparatus. Cytoplasm, perinuclear region. Cell projection. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: P61006 Gene ID: 4218 Database links: Entrez Gene: 4218 Human Entrez Gene: 17274 Mouse Omim: 165040 Human SwissProt: Q5F470 Chicken SwissProt: P61006 Human SwissProt: P55258 Mouse Unigene: 642874 Human Unigene: 709846 Human Unigene: 162811 Mouse Unigene: 16064 Rat |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug Primary: Anti- RAB8A (bsm-51077M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD 
Sample:
Raji(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- RAB8A (bsm-51077M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 25 kD 
Sample:
U937(Human) Cell Lysate at 30 ug Primary: Anti-RAB8A (bsm-51077M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
