| 产品编号 | bsm-51134M |
| 英文名称 | GABARAP |
| 中文名称 | γ-氨基丁酸受体相关蛋白单克隆抗体 |
| 别 名 | ATG8A; FLC 3B; FLC3B; FLJ25768; GABA(A) receptor associated protein; GABA(A) receptor-associated protein; GABARAP a; Gamma aminobutyric acid receptor associated protein; Gamma-aminobutyric acid receptor-associated protein; GBRAP_HUMAN; MGC120154; MGC120155; MM 46; MM46. |
| 研究领域 | 细胞生物 神经生物学 通道蛋白 细胞膜受体 G蛋白信号 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 10B2 |
| 交叉反应 | (predicted: Rabbit,Cow,Rat,Mouse,Human) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human GABARAP protein |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008] Function: May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Subunit: Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1. Interacts with CALR. Interacts with DDX47. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Subcellular Location: Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. Tissue Specificity: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Similarity: Belongs to the MAP1 LC3 family. SWISS: O95166 Gene ID: 11337 Database links: Entrez Gene: 11337 Human Entrez Gene: 56486 Mouse Omim: 605125 Human SwissProt: O95166 Human SwissProt: Q9DCD6 Mouse Unigene: 647421 Human Unigene: 272460 Mouse Unigene: 8411 Rat |
| 产品图片 |
Anti-GABARAP Antibody at 1:1000 dilution + mouse brain lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13918 Da Blocking/Dilution buffer: 5% NFDM/TBST.
Anti- at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
