| 产品编号 | bs-21493R |
| 英文名称 | ARL13B Rabbit pAb |
| 中文名称 | ADP核糖基化因子样蛋白13b抗体 |
| 别 名 | ADP Ribosylation Factor Like GTPase 13B; ADP-Ribosylation Factor-Like 2-Like 1; ARL2-Like Protein 1; ARL2L1; ADP-Ribosylation Factor-Like Protein 2-Like 1; ADP-Ribosylation Factor-Like Protein 13B; ADP-Ribosylation Factor Like GTPase 13B; ADP-Ribosylation Factor-Like 13B; JBTS8; AR13B_HUMAN; |
| 研究领域 | 发育生物学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ARL13B |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] Function: Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic; however, additional evidences are required to confirm these data. Subunit: Monomer (By similarity). Interacts with PIFO. Interacts with IFT complex B components IFT46 and IFT74. Subcellular Location: Plasma membrane Tissue Specificity: Expressed in the developing brain. Post-translational modifications: Sumoylation is required for PKD2 entry into cilium. Similarity: Belongs to the small GTPase superfamily. Arf family. SWISS: Q3SXY8 Gene ID: 200894 Database links: Entrez Gene: 200894 Human Entrez Gene: 68146 Mouse Omim: 608922 Human SwissProt: Q3SXY8 Human SwissProt: Q640N2 Mouse Unigene: 533086 Human Unigene: 96833 Mouse Unigene: 30700 Rat |
| 产品图片 |
Sample: SiHa (Human) Cell Lysate at 30 ug Primary: Anti-ARL13B (bs-21493R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD
Sample:
Cerebrum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ARL13B (bs-21493R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
