| 产品编号 | bs-22203R |
| 英文名称 | Artemin Rabbit pAb |
| 中文名称 | Artemin抗体 |
| 别 名 | ARTN_HUMAN; ARTN; Enovin; EVN; Neublastin; ART; NBN; |
| 研究领域 | 发育生物学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12/19 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Artemin: 141-224/224 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma. [provided by RefSeq, Aug 2016] Function: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Subunit : Homodimer; disulfide-linked. Binds to RET. Subcellular Location: Secreted. Tissue Specificity: Cochlea. Expressed at higher level in sesorineural epithelium than in the modiolus region or substantia nigra. Similarity: Belongs to the TGF-beta family. GDNF subfamily. SWISS: Q9Z0L2 Gene ID: 11876 Database links: Entrez Gene: 9048 Human Entrez Gene: 11876 Mouse Omim: 603886 Human SwissProt: Q5T4W7 Human SwissProt: Q9Z0L2 Mouse Unigene: 632404 Human Unigene: 56897 Mouse Unigene: 154711 Rat 神经生物学相关蛋白（Neurobiology） Artemin 是GDNF 家族的一个新成员，已被证实它是从前的孤儿受体GFRalpha3-RET的配体。 Artemin是感觉和交感神经元培养的一个存活因子，它的表达形式也影响体内的这些神经元。Artemin 还能激活GFRalpha1-RET 复合物并支持培养中脑多巴胺神经元存活 |
| 产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Artemin (bs-22203R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/19 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (bs-22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (bs-22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
