| 产品编号 | bsm-1698M |
| 英文名称 | ox-LDL |
| 中文名称 | 氧化低密度脂蛋白单克隆抗体 |
| 别 名 | ox-LDL: LDL (Copper oxidized); Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein. |
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Specific References (2) | bsm-1698M has been referenced in 2 publications.
[IF=11.413] Mengqi Xu. et al. Osteopontin targeted theranostic nanoprobes for laser-induced synergistic regression of vulnerable atherosclerotic plaques. Acta Pharm Sin B. 2021 Dec;: WB,IF ; Mouse.
[IF=2.447] Yanji Zhu. et al. P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Exp Ther Med. 2022 Feb;23(2):1-8 IHC ; Mouse.
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| 研究领域 | 心血管 细胞生物 免疫学 脂蛋白 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 5C1 |
| 交叉反应 | Rat,Mouse (predicted: Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Full length protein from human plasma |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4. When too much LDL cholesterol circulates in the blood, it can slowly build up in the inner walls of the arteries that feed the heart and brain. Together with other substances it can form plaque, a thick, hard deposit that can clog those arteries. This condition is known as atherosclerosis. Oxidized lipoproteins are formed by free radical damage to lipids that accumulate in macrophages and smooth muscle cells causing foam cell formation, an initial step in the disease. Subcellular Location: Secreted (probable) SWISS: N/A Gene ID: N/A |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ox-LDL) Monoclonal Antibody, Unconjugated (ascites of bsm-1698M 5C1) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ox-LDL) Monoclonal Antibody, Unconjugated (bsm-1698M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
