| 产品编号 | BH0156 |
| 英文名称 | MUC6(ready to use) |
| 中文名称 | 粘蛋白6单克隆抗体（工作液） |
| 别 名 | Gastric mucin 6 ; Gastric Mucin ; Gastric mucin-6 ; MUC 6 ; MUC-6 ; Muc6 ; MUC6 Fragment ; MUC6 mucin ; MUC6 mucin Fragment ; MUC6_HUMAN ; Mucin 6 ; Mucin 6 gastric ; Mucin 6 oligomeric mucus/gel forming ; Mucin glycoprotein Fragment ; Mucin-6 ; Secretory mucin MUC6 Fragment . |
| 研究领域 | 肿瘤 细胞生物 细胞骨架 细胞外基质 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 6H8 |
| 交叉反应 | Human |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 298kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MUC6 |
| 亚 型 | IgG2a |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M PBS(pH7.4) with 1% BSA and 0.1% Proclin300 |
| 保存条件 | Store at 2-8 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. [provided by RefSeq, Dec 2016] Function: May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Play an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis. Subcellular Location: Secreted. Tissue Specificity: Expressed in the regenerative zone of gastric antrum, gastric body mucosa and gastric incisura mucosa. Expressed in the deeper mucous glands of gastric antrum. Overexpressed in Helicobacter pylori infected gastric epithelium. Highly expressed in duodenal Brunner's glands, gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of the common bile duct. Post-translational modifications: O-glycosylated. Similarity: Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 TIL (trypsin inhibitory-like) domain. Contains 3 VWFD domains. SWISS: Q6W4X9 Gene ID: 4588 Database links:
Entrez Gene: 353328 Mouse Omim: 158374 Human SwissProt: Q6W4X9 Human SwissProt: Q80T03 Mouse Unigene: 528432 Human Unigene: 246621 Mouse Unigene: 92457 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC6) Monoclonal Antibody, Unconjugated (BH0156) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
araformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC6) Monoclonal Antibody, Unconjugated (BH0156) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC6) Monoclonal Antibody, Unconjugated (BH0156) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
