| 产品编号 | bsm-52146R |
| 英文名称 | phospho-Cyclin E1 (Thr77) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化周期素E1重组兔单抗 |
| 别 名 | Cyclin E1(T77); Cyclin E1(phospho-Thr77); Cyclin E1(phospho-T77); CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1. |
| 产品类型 | 磷酸化抗体 重组兔单抗 |
| 研究领域 | 肿瘤 细胞生物 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 4G10 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-1000,IHC-F=1:50-1000,IF=1:50-1000,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77: IP(p-T)PD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Function: Essential for the control of the cell cycle at the G1/S (start) transition. Subunit: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Post-translational modifications: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. Similarity: Belongs to the cyclin family. Cyclin E subfamily. SWISS: P24864 Gene ID: 898 Database links: Entrez Gene: 898 Human Entrez Gene: 12447 Mouse Omim: 123837 Human SwissProt: P24864 Human SwissProt: Q61457 Mouse Unigene: 244723 Human Unigene: 16110 Mouse Unigene: 15455 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
