| 产品编号 | D10064 |
| 英文名称 | Microcystin-LR |
| 中文名称 | 微囊藻毒素LR (PP1 and PP2A Inhibitor) |
| 别 名 | 5-l-arginine-cyanoginosinl; 5-l-argininecyanoginosinla; akerstox; microcystin-a; microcystisaeruginosatoxin; Microcystin-LR, Microcystis aeruginosa; MCYST-LR; MCYST-LR; Inhibitor of PP1 and PP2A; PP1 and PP2A Inhibitor. 微囊藻毒素; 微囊藻毒素LR; 快速死亡因子; 铜绿微囊藻; PP1 and PP2A抑制剂; 微囊藻毒素(LR亚型); |
| 克 隆 号 | |
| CAS | 101043-37-2 |
| 理论分子量 | kDa |
| 检测分子量 | |
| 保存条件 | Store at -20℃ stable for at least 12 months. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
基本信息： CAS：101043-37-2 分子式：C49H74N10O12 分子量：995.17 纯度：≥95% 外观/性状：固体薄膜 溶解性：溶于甲醇，DMSO 敏感性：易吸潮 产品简介： 从淡水蓝藻铜绿微囊藻中分离出的环七肽毒素。蛋白磷酸酶1（pp1）和蛋白磷酸酶2a（pp2a）的有效抑制剂。不像秋葵酸，微囊藻毒素lr对PP1（IC50=1.7 nM）和PP2A（IC50=40 PM）均有效。以两步反应机制与磷酸酶反应，包括催化亚单位的快速结合和失活，以及较慢的共价相互作用。对蛋白激酶无影响，有助于降低蛋白激酶检测中污染磷酸酶活性的影响。微囊藻毒素lr，铜绿微囊藻溶于DMSO中也可用。 产品用途： 微囊藻毒素-lr，铜绿微囊藻是一种有效的蛋白磷酸酶1（pp1）和蛋白磷酸酶2a（pp2a）抑制剂。primary target IC50：1.7 nM and 40 pM against PP1 and PP2A, respectively 体外研究： Microcystins are toxic molecules produced by cyanobacterial blooms due to water eutrophication. Microcystin-LR proves to be a potent inhibitor of type 1 (IC50=1.7 nM) and type 2A (IC50= 0.04 nM) protein phosphatases. Microcystin-LR inhibits the activity of both type 1 and type 2A phosphatases >10-fold more potently than okadaic acid under the same conditions. Type 2A protein phosphatases in dilute mammalian cell extracts are found to be completely inhibited by 0.5 nM microcystin-LR while type 1 protein phosphatases are only slightly affected at this concentration. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
