| 产品编号 | Abk004 |
| 英文名称 | Tumor Immunity Characterization Antibody Assembly kit |
| 中文名称 | 肿瘤免疫表征分析抗体套装 |
| 别 名 | Tumor immunity Characterization Antibody Assembly kit(CD4,CD8,PD-1,PD-L1,CTLA4) 肿瘤免疫表征分析抗体套装(CD4,CD8,PD-1,PD-L1,CTLA4) |
| 研究领域 | 肿瘤 细胞生物 免疫学 t-淋巴细胞 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Recent clinical advances and basic discoveries have triggered a revolution in cancer immunotherapy. As a great breakthrough, some key immune checkpoints on the surface of tumor or immune cells were shown to play important roles in tumor immunity regulation and intervention of these targets will be beneficial to tumor therapy. Characterization of tumor infiltrating immune cells and tumor cells by examining immune cell phenotyping markers and immuno-checkpoints is important to efficacy of cancer immunotherapy. Among them, canonical helper T cell marker CD4, cytotoxic T cell marker CD8, PD-1 receptor on the surface of T cells, PD-1 ligand PD-L1 and CTLA-4 on the surface of tumor cells are top targets to be evaluated in tumor immunity research by different applications such as immuno-histochemistry (IHC) or Flow Cytometry (FCM). Tumor Immunity Characterization Antibody Assembly kit is one economical package of selected antibodies against CD4, CD8,PD-1, PD-L1 and CTLA4 respectively. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Tumor Immunity Characterization Antibody Assembly kit Rabbit Anti-CD4 antibody (bs-0647R) Rabbit Anti-CD8 antibody (bs-0648R) Rabbit Anti-PD-1 antibody (bs-1867R) Rabbit Anti-PD-L1 antibody (bs-4941R) Rabbit Anti-CTLA4 antibody (bs-10006R) SWISS: N/A Gene ID: N/A Database links: Tumor Immunity Characterization Antibody Assembly kit Rabbit Anti-CD4 antibody (bs-0647R) Rabbit Anti-CD8 antibody (bs-0648R) Rabbit Anti-PD-1 antibody (bs-1867R) Rabbit Anti-PD-L1 antibody (bs-4941R) Rabbit Anti-CTLA4 antibody (bs-10006R)
基础研究的不断进步和最近的临床进展引发了一场癌症免疫治疗的革命。作为一项重大突破，肿瘤或免疫细胞表面的一些关键免疫检查点分子在肿瘤免疫调节中发挥着重要作用，对这些靶点进行干预将有利于肿瘤的治疗。通过检测免疫细胞表面标志和多种免疫检查点的表达情况对肿瘤浸润免疫细胞和肿瘤细胞表征进行分析，对肿瘤免疫治疗的疗效有重要意义。其中，经典的辅助性T细胞标志CD4、细胞毒性T细胞标志CD8、T细胞表面表达的PD-1受体、肿瘤细胞表面表达的PD-L1（PD-1配体）和CTLA-4是在肿瘤免疫研究中利用免疫组织化学(IHC)或流式细胞术(FCM)进行分析的重要指标。肿瘤免疫表征分析抗体套装极具性价比，包含了分别用于检测CD4、CD8、PD-1、PD-L1和CTLA 4的抗体。这些抗体在多种应用和物种中进行了测试。有关抗体具体使用信息，请参阅各个抗体产品数据表。 |
| 产品图片 |
Primary: Anti- CD4(bs-0647R)
Sample: HL60 Cell Lysate at 40 ug Primary: Anti- CD4(bs-0647R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55kD 
Primary: Anti-PD-L1 (bs-4941R)
Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-PD-L1(bs-4941R)at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD 
Primary: Anti-PD-1 (bs-1867R)
Sample: Raji(Human) Cell Lysate at 30 ug Thymus(Mouse) Lysate at 40 ug Lymph node(Mouse) Lysate at 40 ug Spleen(Mouse) Lysate at 40 ug Raw264.7(Mouse) Cell Lysate at 40 ug Thymus (Rat) Lysate at 40 ug Primary: Anti-PD-1 (bs-1867R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 58 kD 
Primary: Anti-CD8(bs-0648R)
Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- CD8(bs-0648R)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 36 kD 
Primary: Anti-CTLA4(bs-10006R)
Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CTLA4 (bs-10006R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
