产品编号 | bs-48004P |
英文名称 | Recombinant human TNF-Alpha protein (Active, HEK293) |
中文名称 | 重组人肿瘤坏死因子α (活性的, Tag free) |
别 名 | Tumor necrosis factor-α; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a ; TNF alpha; TNFalpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha. ;Recombinant human TNF-Alpha protein (Active, HEK293) 肿瘤坏死因子α重组蛋白; 肿瘤坏死因子α; 重组人肿瘤坏死因子α; 重组人TNF α; |
理论分子量 | 17kDa |
检测分子量 | 17 kDa |
性 状 | Lyophilized powder |
浓 度 | >0.5 mg/ml |
物 种 | Human |
序 列 | 77-233/233 |
纯 度 | >95% as determined by SDS-PAGE |
纯化方法 | AC |
内毒素 | <0.5 EU/μg as determined by LAL. |
表达系统 | HEK293 cell |
活性 | Yes |
标签 | Tag free |
缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Tumor necrosis factor alpha (TNFα) is an EGF related polypeptide growth factor that signals through the TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and also produced by monocytes, keratinocytes, and various tumor cells. TNFα stimulates the proliferation of a wide range of epidermal and epithelial cells. TNFα is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation, impair regulatory T cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. This recombinant human TNF alpha (Glycosylated homotrimer) is produced by human cells.(Tag free) Biological activity: The activity was measured by the cytotoxicity in L-929 cell in the presence of actinomycin D. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P01375 Gene ID: 7124 |
产品图片 | |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |