产品编号 | bs-48005P |
英文名称 | Recombinant human IL-10 protein (Active, HEK293) |
中文名称 | 重组人白介素10 (活性的, Tag free) |
别 名 | Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重组蛋白; 人白细胞介素10 ; 重组人白介素10; 重组人IL-10; |
理论分子量 | 17kDa |
检测分子量 | 21 kDa |
性 状 | Lyophilized powder |
浓 度 | >0.5 mg/ml |
物 种 | Human |
序 列 | 19-178/178 |
纯 度 | >95% as determined by SDS-PAGE |
纯化方法 | AC |
内毒素 | <0.5 EU/μg as determined by LAL. |
表达系统 | HEK293 cell |
活性 | Yes |
标签 | Tag free |
缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. This recombinant human IL-10 is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P18893 Gene ID: 3586 |
产品图片 | |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |