产品编号 | bs-48017P |
英文名称 | Recombinant human BMP-7 protein (Active, HEK293) |
中文名称 | 重组人骨形态发生蛋白-7 (活性的, Tag free) |
别 名 | rhBMP-7; Bone morphogenetic protein 7 precursor; osteogenic protein 1; BMP7; OP-1; BMP 7; Bone morphogenic protein 7; Eptotermin alfa; OP 1; OP1; BMP7_HUMAN. ;Recombinant human BMP-7 protein (Active, HEK293) 人骨形态发生蛋白-7重组蛋白; 重组人骨形态发生蛋白-7; 人重组骨形态发生蛋白-7; 重组人BMP7; |
检测分子量 | 30-38 kDa |
性 状 | Lyophilized powder |
浓 度 | >0.5 mg/ml |
物 种 | Human |
序 列 | 293-431/431 |
纯 度 | >95% as determined by SDS-PAGE |
纯化方法 | AC |
内毒素 | <0.5 EU/μg as determined by LAL. |
表达系统 | HEK293 cell |
活性 | Yes |
标签 | Tag free |
缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP1) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-7 plays an important role in cartilage and bone formation. It also plays a role in calcium regulation and kidney development. Mature human BMP-7 is usually a homodimer consisting of two antiparallel monomers of 139 amino acids. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif. This recombinant human BMP-7 is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P18075 Gene ID: 655 |
产品图片 | |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |