| 产品编号 | bs-48019P |
| 英文名称 | Recombinant human VEGF-121 protein (Active, HEK293) |
| 中文名称 | 重组人VEGF121 (活性的, Tag free) |
| 别 名 | rhVEGF121; Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor; Recombinant human VEGF-121 protein (Active, HEK293) 人VEGF121; 人VEGF121重组蛋白; 重组人VEGF121; 人重组人VEGF121; 重组人血管内皮生长因子121; |
| 检测分子量 | 30-40 kDa |
| 性 状 | Lyophilized powder |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 1-232/232 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Vascular endothelial growth factor (VEGF ) is a potent growth and angiogenic cytokine. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure .VEGF stimulates angiogenesis, asculogenesis and endothelial cell growth, induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Human express alternately spliced isoforms, and VEGF-121 is the only form that lacks a basic heparinbinding region and is freely diffusible. VEGF-121 induces the proliferation of lymphatic endothelial cells and the lymph angiogenesis may be promoted by up regulation of VEGF-121. This recombinant human VEGF-121 is produced by human cells. (Tag free) Mol wt: 37kDa homodimer, 50kDa homotrimer Biological activity: The activity was measured by its ability to stimulate the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P15692 Gene ID: 7422 |
| 产品图片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
