| 产品编号 | bs-48021P |
| 英文名称 | Recombinant human EPO protein (Active, HEK293) |
| 中文名称 | 重组人促红细胞生成素 (活性的, Tag free) |
| 别 名 | EPO_HUMAN; Erythropoietin; Epoetin; EP; DBAL; ECYT5; MVCD2; 人促红细胞生成素; 人促红细胞生成素重组蛋白; 重组人促红细胞生成素; 人重组人促红细胞生成素; 重组人EPO; |
| 克 隆 号 | |
| 理论分子量 | 36 kDa |
| 检测分子量 | 36-40 |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 28-193/193 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Recombinant human erythropoietin(EPO) expressed in engineered human cells. EPO belongs to the EPO/TPO protein family. EPO is an acidic hormone mainly produced by kidneys. EPO promotes erythroid progenitor cell differentiation and initiates hemoglobin synthesis. EPO acts by binding to a specific erythropoietin receptor (EPOR) present on the red blood cell precursor in the bone marrow, stimulating these cells to transform into mature erythrocytes. The EPO sensitivity progressively increases with differentiation of immature progenitor cells. This recombinant human EPO is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). EPO activity is 79KU/mg. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 产品图片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
