| 产品编号 | bs-48023P |
| 英文名称 | Recombinant human FLT3 Ligand protein (Active, HEK293) |
| 中文名称 | 重组人FLT3 Ligand (活性的, Tag free) |
| 别 名 | rhFLT3 Ligand; Flt 3 ligand; Flt 3L; Flt3 L; FLT3 LG; Flt3 ligand; Flt3L; FLT3L_HUMAN; FLT3LG; Fms related tyrosine kinase 3 ligand; Fms-related tyrosine kinase 3 ligand; SL cytokine. ;Recombinant human FLT3 Ligand protein (Active, HEK293) 人FLT3 Ligand重组蛋白; 重组人FLT3 Ligand; 人重组FLT3 Ligand; 重组人FLT3 Ligand; |
| 检测分子量 | 24-30 kDa |
| 性 状 | Lyophilized powder |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 27-185/235 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Human Flt3 Ligand is a 24-30kDa glycoprotein with a 158 amino acid. Flt3 Ligand is expressed by T cells, bone marrow and thymic fibroblasts. The predominant biologically active form is membranebound isoform, which can be proteolytically cleaved to generate a biologically active soluble isoform. Flt3 Ligand synergizes well with a number of other colony stimulating factors and interleukins to regulate proliferation of early hematopoietic cells by activating Flt3. This recombinant human FLT3 Ligand is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of the human acute myeloid leukemia cell line OCI-AML5. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P49771 Gene ID: 2323 |
| 产品图片 |
The purity of the protein is greater than 95% as determined by reduced and non reduced SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
