| 产品编号 | bs-48027P |
| 英文名称 | Recombinant human IL-3 protein (Active, HEK293) |
| 中文名称 | 重组人IL-3 (活性的, Tag free) |
| 别 名 | rhIL-3; Colony stimulating factor multiple; Hematopoietic growth factor; IL 3; IL3; IL3_HUMAN; Interleukin 3 (colony stimulating factor, multiple); Interleukin 3; Interleukin-3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; Multipotential colony stimulating factor; Multipotential colony-stimulating factor; OTTHUMP00000065963; P cell stimulating factor; P-cell-stimulating factor. ;Recombinant human IL-3 protein (Active, HEK293) 人IL-3重组蛋白; 重组人IL-3; 人重组IL-3; 重组人IL-3; 白细胞介素3; 白介素3; |
| 理论分子量 | 19kDa |
| 检测分子量 | 25-40 kDa |
| 性 状 | Lyophilized powder |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 20-139/152 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Interleukin-3 (IL-3) is belongs to hematopoietic growth factors. It is a secreted protein. IL-3 can improve the body's natural response to disease as part of the immune system. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells (as opposed to lymphoid progenitor cells where differentiation is stimulated by IL-7) as well as stimulates proliferation of all cells in the myeloid lineage (erythrocytes, megakaryocytes, granulocytes, monocytes, and dendritic cells) in conjunction with other cytokines, such as EPO, GM-CSF, TPO and IL-6. Additionally, IL-3 aids in embryo implantation and placental development, was found to be decreased both in pregnant women with antiphospholipid syndrome and in animal models of APS. IL-3 also participates in the response of the organism to various types of stress. This recombinant human IL-3 is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P08700 Gene ID: 3562 |
| 产品图片 |
The purity of the protein is greater than 95% as determined by reduced and non reduced SDS-PAGE
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
