| 产品编号 | bs-41230P |
| 英文名称 | Tags Positive Control Whole Cell Lysate, His |
| 中文名称 | 标签阳性对照 (11 Tags, 41kDa)全细胞裂解液 |
| 别 名 | Tags Positive Control (T7; HSV; C-Myc; VSV-g; Glu-Glu; V5; E-tag; FLAG (DDDDK); S-tag; HA; His; GST); E. coli Tags Positive Control Whole Cell Lysate; E. coli Positive Control (Escherichia coli) Whole Cell Lysate; E. coli Tags Positive Control (Escherichia coli) Whole Cell Lysate; E.coli Tags Positive Control Whole Cell Lysate; Escherichiacoli Tags Positive Control Whole Cell Lysate; epitope tags; E. coli Positive Control (Escherichia coli ) Whole Cell Lysate; ;Tags Positive Control Whole Cell Lysate, His 重组标签蛋白 (11 Tags, 41kDa); 标签蛋白阳性对照品; 大肠杆菌多标签阳性对照裂解液; 大肠杆菌多标签阳性对照全细胞裂解液; 大肠杆菌Tags阳性对照（大肠埃希氏菌）全细胞裂解液; 大肠杆菌11 Tags(41kDa)阳性对照全细胞裂解液; 大肠杆菌阳性对照（大肠杆菌）全细胞裂解物; |
| 克 隆 号 | |
| 理论分子量 | 41 kDa |
| 检测分子量 | 41 |
| 性 状 | Liquid |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | N/A |
| 序 列 | GST tag & T7 tag & HSV tag & Myc & VSV-G tag & Glu-Glu tag & V5 tag & FLAG & S-tag & HA & 8×His |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | Not analyzed |
| 表达系统 | E.coli |
| 活性 | Not tested |
| 标签 | His |
| 缓 冲 液 | 10mM TBS (pH7.4) with 0.02% Proclin300. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of: T7 HSV C-Myc VSV-g Glu-Glu V5 E-tag FLAG (DDDDK) S-tag HA 6x His GST Tag sequences: T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC VSV-g = YTDIEMNRLGK Glu-Glu = EYMPME V5 = GKPIPNPLLGLDST E-tag = GAPVPYPDPLEPR FLAG = DYKDDDDK HA = YPYDVPDYAC 6xHIS = HHHHHH Lyophilised: After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times. Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags. |
| 产品图片 |
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
