| 产品编号 | D10376 |
| 英文名称 | Lysozyme from chicken egg white |
| 中文名称 | 溶菌酶 (≥40KU/mg, 进口) |
| 别 名 | Mucopeptide N-acetylmuramoylhydrolase, Muramidase; lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein; 溶解酵素; 溶菌酶 来源于鸡蛋白; 胞壁质酶; 粘肽 N-乙酰胞壁质水解酶; |
| 克 隆 号 | |
| CAS | 12650-88-3 |
| 理论分子量 | kDa |
| 检测分子量 | |
| 保存条件 | Store at -20℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
CAS号：12650-88-3 分子量：single-chain 14.3kDa MDL：MFCD00131557 级别/活性：BR,≥40,000 units/mg protein 纯度：≥90% (HPLC) 外观/性状：lyophilized powder 酶活性定义：One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path). Biochem/physiol Actions: Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M). L6876 (Sigma-Aldrich) |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
