| 产品编号 | bsm-51615M |
| 英文名称 | RAB1B |
| 中文名称 | RAB1B单克隆抗体 |
| 别 名 | RAB1B member RAS oncogene family; RAB1B_HUMAN; Ras related protein Rab1B; Ras-related protein Rab-1B; Small GTP binding protein. |
| 研究领域 | 信号转导 G蛋白信号 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | C7D12 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human RAB1B between 1-201 amino acids. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009] Function: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Subcellular Location: Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm. Post-translational modifications: Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: Q9H0U4 Gene ID: 81876 Database links: Entrez Gene: 81876 Human SwissProt: Q9H0U4 Human |
| 产品图片 |
Sample:
Lane 1: Hela cell lysates Lane 2: A431 cell lysates Lane 3: U-20S cell lysates Lane 4: Rat liver tissue lysates Lane 5: NIH/3T3 cell lysates Primary: Anti-RAB1B (bsm-51615M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 25 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
