| 产品编号 | bsm-51625M |
| 英文名称 | CBFA2T3 |
| 中文名称 | CBFA2T3单克隆抗体 |
| 别 名 | Ccore binding factor runt domain alpha subunit 2 translocated to 3; ETO2; hMTG16; hMTG16a; hMTG16b; MTG16; MTG16_HUMAN; MTG8 related gene 2; MTG8 related protein MTG16a; MTG8 related protein MTG16b; MTG8-related protein 2; MTGR2; Myeloid translocation gene 8 and 16b; Myeloid translocation gene on chromosome 16 protein; Protein CBFA2T3; Zinc finger MYND domain-containing protein 4; ZMYND4. |
| 研究领域 | 肿瘤 染色质和核信号 表观遗传学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | F12T3 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CBFA2T3 between 418-653 amino acids. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. Function: Functions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation. Isoform 2 functions as an A-kinase-anchoring protein. Subcellular Location: Nucleus > nucleolus. The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm and Nucleus > nucleoplasm. Golgi apparatus membrane. Tissue Specificity: Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level). Similarity: Belongs to the CBFA2T family. Contains 1 MYND-type zinc finger. Contains 1 TAFH (NHR1) domain. SWISS: O75081 Gene ID: 863 Database links: Entrez Gene: 863 Human SwissProt: O75081 Human |
| 产品图片 |
Sample:
Lane 1: Jurkat cell lysates Lane 2: CCRF-CEM cell lysates Primary: Anti-CBFA2T3 (bsm-51625M) at 1/1000~2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
