| 产品编号 | bsm-51636M |
| 英文名称 | USP2 |
| 中文名称 | 泛素羧基末端水解酶2单克隆抗体 |
| 别 名 | 41 kDa ubiquitin specific protease ; 41 kDa ubiquitin-specific protease ; Deubiquitinating enzyme 2 ; Ubiquitin carboxyl terminal hydrolase 2 ; Ubiquitin carboxyl-terminal hydrolase 2 ; ubiquitin specific peptidase 2 ; Ubiquitin specific processing protease 2 ; Ubiquitin specific protease 12 ; Ubiquitin specific protease 9 ; Ubiquitin specific protease, 41kD ; Ubiquitin thioesterase 2 ; Ubiquitin thiolesterase 2 ; Ubiquitin-specific-processing protease 2 ; Ubiquitin-specific-processing protease testis ; UBP-t ; UBP2_HUMAN ; UBP41 ; USP9 |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 泛素 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | K08F1 |
| 交叉反应 | Human,Monkey |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 68kDa |
| 检测分子量 | 72 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human USP2 |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Subcellular Location: Cytoplasm and Cytosol Tissue Specificity: Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level). SWISS: O75604 Gene ID: 9099 Database links:
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| 产品图片 |
Sample:
Lane 1: MCF-7 cell lysates Lane 2: PC-3 cell lysates Lane 3: COS-7 cell lysates Lane 4: Du145 cell lysates Lane 5: SK-BR-3 cell lysates Primary: Anti-USP2 (bsm-51636M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 72 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
