| 产品编号 | bsm-51639M |
| 英文名称 | PACSIN2 |
| 中文名称 | 胞浆磷蛋白PACSIN2单克隆抗体 |
| 别 名 | cytoplasmic phosphoprotein PACSIN2; Moderately similar to h74 gene product; OTTHUMP00000198281; OTTHUMP00000198282; OTTHUMP00000198283; OTTHUMP00000198284; PACN2_HUMAN; protein kinase C and casein kinase substrate in neurons 2; protein kinase C and casein kinase substrate in neurons 2; Protein kinase C and casein kinase substrate in neurons protein 2; Protein kinase C and casein kinase substrate in neurons protein; SDP II; SDPII; Syndapin II. |
| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | H5F12 |
| 交叉反应 | Human (predicted: Mouse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PACSIN2 between 250-486 amino acids. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Function: May play a role in vesicle formation and transport. Subunit: Homodimer. May form heterooligomers with other PACSINs.Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4. Subcellular Location: Cytoplasmic vesicle. Vesicle-like cytoplasmic distribution. Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). Similarity: Belongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain. SWISS: Q9UNF0 Gene ID: 11252 Database links: Entrez Gene: 11252 Human Entrez Gene: 23970 Mouse SwissProt: Q9UNF0 Human SwissProt: Q9WVE8 Mouse |
| 产品图片 |
Sample:
Lane 1: Daudi cell lysates Lane 2: PC-3 cell lysates Lane 3: Human brain tissue lysates Lane 4: Mouse brain tissue lysates Primary: Anti-PACSIN2 (bsm-51639M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 58 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
