| 产品编号 | bsm-51641M |
| 英文名称 | FNDC5 |
| 中文名称 | FNDC5单克隆抗体 |
| 别 名 | Fibronectin type III domain containing 5; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat containing protein 2; Fibronectin type III repeat-containing protein 2; FNDC 5; FNDC5_HUMAN; FRCP2; Irisin. |
| 研究领域 | 细胞生物 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | F8U6 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12/20kDa |
| 细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FNDC5: 51-150/212 <Extracellular> |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. Function: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein. Note=Imported in peroxisomes through the PEX5 receptor pathway. Irisin: Secreted. Note=Detected in the blood of individuals subjected to endurance exercise. Post-translational modifications: Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated. Similarity: Contains 1 fibronectin type-III domain.. SWISS: Q8NAU1 Gene ID: 252995 Database links: Entrez Gene: 252995 Human SwissProt: Q8NAU1 Human |
| 产品图片 |
Sample:
Lane 1: Human skeletal muscle tissue lysates Primary: Anti-FNDC5 (bsm-51641M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 12/20kD Observed band size: 22 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
