| 产品编号 | bsm-51648M |
| 英文名称 | MMP1 |
| 中文名称 | 基质金属蛋白酶-1单克隆抗体 |
| 别 名 | 27 kDa interstitial collagenase; CLGN; CLG; collagenase, fibroblast; Fibroblast collagenase; Interstitial collagenase; MMP 1; MMP-1; MMP; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix metalloproteinase-1; MMP1_HUMAN; OTTHUMP00000045866; Matrix Metalloproteinase 1. |
| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | T6R1 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27/41/54kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human MMP-1. |
| 亚 型 | IgG2a |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009] Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Subunit: Interacts with HIV-1 Tat. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Post-translational modifications: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P03956 Gene ID: 4312 Database links: Entrez Gene: 4312 Human SwissProt: P03956 Human |
| 产品图片 |
Sample:
Lane 1: Raji cell lysates Lane 2: PC-3 cell lysates Lane 3: MDA-MB-231 cell lysates Primary: Anti-MMP1 (bsm-51648M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 27/41/54 kD Observed band size: 54 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
