| 产品编号 | bsm-51656M |
| 英文名称 | EIF2B3 |
| 中文名称 | eIF2Bγ单克隆抗体 |
| 别 名 | EI2BG_HUMAN; EIF 2B; EI2BG; eIF 2B GDP GTP exchange factor subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma; eIF2B-gamma; EIF2Bgamma; Eukaryotic translation initiation factor 2B subunit 3 gamma; Eukaryotic translation initiation factor 2B, gamma; eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa; OTTHUMP00000010262; OTTHUMP00000010263; Translation initiation factor eIF-2B subunit gamma. |
| 研究领域 | 细胞生物 神经生物学 表观遗传学 Alzheimer's |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | H11F4 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:25, ICC=1:25, IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EIF2B3: 151-400/452 |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Function: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Subunit: Complex of five different subunits; alpha, beta, gamma, delta and epsilon. DISEASE: Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Similarity: Belongs to the eIF-2B gamma/epsilon subunits family. SWISS: Q9NR50 Gene ID: 8891 Database links: Entrez Gene: 8891 Human SwissProt: Q9NR50 Human |
| 产品图片 |
Sample:
Lane 1: HL-60 cell lysates Lane 2: MCF-7 cell lysates Lane 3: U-2OS cell lysates Lane 4: K562 cell lysates Primary: Anti-EIF2B3 (bsm-51656M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 56 kD 
U-2 OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EI2BG) monoclonal Antibody, Unconjugated (bsm-51656M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Dylight® 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
