| 产品编号 | bsm-51669M |
| 英文名称 | Adenylate Kinase 1 |
| 中文名称 | 腺苷酸激酶-1单克隆抗体 |
| 别 名 | AK 1; AK1; AK-1; KAD1_HUMAN; Adenylate kinase isoenzyme 1; Adenylate kinase soluble; ATP AMP transphosphorylase; Myokinase; ATP-AMP transphosphorylase 1. |
| 研究领域 | 肿瘤 心血管 信号转导 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | G6E1 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-4000, IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human AK1 between 1-193 amino acids. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Function: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Subunit: Monomer. Subcellular Location: Cytoplasm. DISEASE: Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD) [MIM:612631]. Similarity: Belongs to the adenylate kinase family. SWISS: P00568 Gene ID: 203 Database links: Entrez Gene: 203 Human SwissProt: P00568 Human |
| 产品图片 |
Sample:
Lane 1: 293 cell lysates Lane 2: HepG2 cell lysates Lane 3: Human testis tissue lysates Primary: Anti-Adenylate Kinase 1 (bsm-51669M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 24 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
