| 产品编号 | bsm-51687M |
| 英文名称 | PU.1/Spi1 |
| 中文名称 | 造血转录因子PU.1单克隆抗体 |
| 别 名 | 31 kDa Transforming Protein; 31 kDa-transforming protein; Hematopoietic transcription factor PU.1; OF; SFPI1; SPI 1 proto oncogene; SPI A; SPI1_HUMAN; Spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1. |
| 研究领域 | 免疫学 转录调节因子 b-淋巴细胞 表观遗传学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | K8J9 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human SPI1 |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq, Oct 2008] Function: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. Subunit: Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Subcellular Location: Nucleus. Similarity: Belongs to the ETS family. Contains 1 ETS DNA-binding domain. SWISS: P17947 Gene ID: 6688 Database links: Entrez Gene: 6688 Human SwissProt: P17947 Human |
| 产品图片 |
Sample:
Lane 1: K562 cell lysates Lane 2: Daudi cell lysates Lane 3: THP-1 cell lysates Primary: Anti-PU.1/Spi1 (bsm-51687M) at 1/500~1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 40 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
