| 产品编号 | bsm-51690M |
| 英文名称 | DKK2 |
| 中文名称 | DKK2单克隆抗体 |
| 别 名 | Dickkopf 2; Dickkopf gene 2; Dickkopf homolog 2; Dickkopf related protein 2; Dickkopf-2; Dickkopf-related protein 2; Dickkopf2; DKK 2; Dkk-2; DKK2_HUMAN; hDkk 2; hDkk-2; hDkk2. |
| 研究领域 | 干细胞 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | J3F5 |
| 交叉反应 | Human (predicted: Mouse) |
| 产品应用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human DKK2. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008] Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Subunit: Interacts with LRP5 and LRP6. Subcellular Location: Secreted. Tissue Specificity: Expressed in heart, brain, skeletal muscle and lung. Post-translational modifications: May be proteolytically processed by a furin-like protease. Similarity: Belongs to the dickkopf family. SWISS: Q9UBU2 Gene ID: 27123 Database links: Entrez Gene: 27123 Human Entrez Gene: 56811 Mouse SwissProt: Q9UBU2 Human SwissProt: Q9QYZ8 Mouse |
| 产品图片 |
Sample:
Lane 1: Human brain tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-DKK2 (bsm-51690M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 26 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
