| 产品编号 | bsm-51716M |
| 英文名称 | RNF4 |
| 中文名称 | RNF4单克隆抗体 |
| 别 名 | RNF4_HUMAN; E3 ubiquitin ligase RNF4; E3 ubiquitin protein ligase RNF4; Protein SNURF; RES4 26; RING finger protein 4; SLX5; Small nuclear ring finger protein; SNURF. |
| 研究领域 | 染色质和核信号 表观遗传学 泛素 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | E4R2 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human RNF4. |
| 亚 型 | IgG1,κ |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010] Function: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation. Subcellular Location: Cytoplasm. Nucleus. Nucleus. PML body. Nucleus. nucleoplasm. Tissue Specificity: Widely expressed at low levels in many tissues; highly expressed in testis. Post-translational modifications: Sumoylated; conjugated by one or two SUMO1 moieties. Autoubiquitinated. Similarity: Contains 1 RING-type zinc finger. SWISS: P78317 Gene ID: 6047 Database links: Entrez Gene: 51573 Human Omim: 605943 Human SwissProt: Q9NZC3 Human Unigene: 512607 Human |
| 产品图片 |
Sample:
Lane 1: LNCap cell lysates Lane 2: PC-3 cell lysates Lane 3: Human testis tissue lysates Primary: Anti-RNF4 (bsm-51716M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 34 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
