| 产品编号 | bsm-51742M |
| 英文名称 | GSTA1 |
| 中文名称 | GSTA1单克隆抗体 |
| 别 名 | Glutathione S alkyltransferase A1; Glutathione S aralkyltransferase A1; Glutathione S aryltransferase A1; Glutathione S transferase 2; Glutathione S transferase A1; Glutathione S transferase alpha 1; Glutathione S transferase Ha subunit 1; Glutathione S-transferase A1; GST 2; GST class alpha; GST class alpha 1; GST class alpha member 1; GST class-alpha member 1; GST epsilon; GST HA subunit 1; GST, class alpha, 1; GST-epsilon; GST2; GSTA 1; GSTA1 1; GSTA1 protein; GSTA1-1; GSTA1_HUMAN; GTH 1; GTH1; HA subunit 1; MGC131939; OTTHUMP00000016611; S (hydroxyalkyl)glutathione lyase A1. |
| 研究领域 | 肿瘤 信号转导 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | A12K9 |
| 交叉反应 | Human,Rat |
| 产品应用 | WB=1:1000-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human GSTA1. |
| 亚 型 | IgG1,κ |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Members of the glutathione S-transferase (GST) family of proteins function in the detoxification of xenobiotics to protect cells against toxicant-induced damage. GSTs are differentially expressed in lung, liver and kidney tissue. Three isoforms, GSTA1-1, GSTA1-4 and GSTM1, localize to the mitochondria in addition to the cytoplasm. In normal and transformed cells, the oncoprotein Myb transcriptionally upregulates GSTM1. This isoform shows high specific activity for aflatoxin B1 epoxide conjugation, suggesting an important role for this interaction in the defense against both chemical and oxidative stress. The C-terminal domain of GSTA1 may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity. Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Subunit: Homodimer or heterodimer of GSTA1 and GSTA2. Subcellular Location: Cytoplasm. Tissue Specificity: Liver. Post-translational modifications: S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. Similarity: Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. SWISS: P08263 Gene ID: 2938 Database links: Entrez Gene: 2938 Human SwissProt: P08263 Human |
| 产品图片 |
Sample:
Lane 1: HepG2 cell lysates Lane 2: Caco2 cell lysates Lane 3: LNCap cell lysates Lane 4: Rat liver tissue lysates Primary: Anti-GSTA1 (bsm-51742M) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
