| 产品编号 | bs-47080P |
| 英文名称 | Recombinant human CTLA4 protein, C-His (HEK293) |
| 中文名称 | 重组人CTLA4蛋白 |
| 别 名 | CD 152; CD152; CD152 antigen; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte-associated protein 4; GSE; IDDM12; CD152 isoform; CTLA4_HUMAN; GRD4; ICOS; Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; Recombinant human CTLA4 protein, C-His (HEK293) |
| 克 隆 号 | |
| 理论分子量 | 14 kDa |
| 检测分子量 | Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result. |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 36-161/223 |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 纯化方法 | AC |
| 内毒素 | <1.0 EU/μg as determined by LAL |
| 表达系统 | HEK293 cell |
| 活性 | Not tested |
| 标签 | C-His |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 | |
| 产品图片 |
Immobilized Human CTLA4,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human CD86,hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
Immobilized Human CD80, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human CTLA4, His Tag with the EC50 of 82.31ng/ml determined by ELISA.
The purity of Human CTLA4 is greater than 95% as determined by SEC-HPLC.
Human CTLA4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
